BLR is a recA- derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences or whose products may cause the loss of the DE3 prophage (1, 2).
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
Genotype: F-ompT hsdSB(rB- mB-) gal lac ile dcm Δ(srl-recA)i306::Tn10 (tetR)(DE3)
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BLR is a recA derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences.
0.4 ml in Plastic ampoule
1 ml in Glass bottle
0.4 ml1 mlComponent
•2 × 0.2 ml5 × 0.2 mlBLR(DE3) Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•2 ng2 ngTest Plasmid
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
1. A. Roca (U. of Wisconsin), personal
2. Studier, F. W. (1991) J. Mol. Biol. 219,
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