684274

β1,2-Xylosidase, Xanthomonas sp.

• Synonym(s): β1,2-Xylosidase, Xanthomonas sp., β-D-Xylanxylohydrolase
• CAS Number: 9025-53-0
• Enzyme Commission number: 3.2.1.37 (BRENDA, IUBMB)
• MDL number: MFCD00130640
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• form: liquid
• Quality Level: 100
• specific activity: ≥10 units/mL; ≥20 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• foreign activity: β-N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α-mannosidase, proteases, none detected
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Native β1,2-Xylosidase from Xanthomonas species. Catalyses the hydrolysis of terminal xylose β1,2-linked to the β-mannose of N-linked oligosaccharides. Enzyme requires removal of a1,3-linked mannose. Enzyme requires 5 mM calcium chloride in reaction buffer for full activity.

Native β1,2-Xylosidase from Xanthomonas species. Catalyzes the hydrolysis of terminal xylose β1,2-linked to the β-mannose of N-linked oligosaccharides. Enzyme requires removal of α1,3-linked mannose. Enzyme requires 5 mM calcium chloride in reaction buffer for full activity.

Note: 1 mU = 1 milliunit.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will release 1 nmol of methylumbelliferone from 4-methylumbelliferyl-7-β-D-xyloside per min at 37°C, pH 5.0.

Physical form

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

Reconstitution

Following initial use, refrigerate (4°C).

Other Notes

Reese, A.J. and Doening, T.L. 2003. Mol. Microbiol.50, 1401.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable