605195

Thrombin, Human Plasma, High Activity

• Synonym(s): Thrombin, Human Plasma, High Activity
• CAS Number: 9002-04-4
• UNSPSC Code: 12352202
• NACRES: NA.25

Properties

• biological source: human plasma
• Quality Level: 100
• form: lyophilized solid
• specific activity: ≥2800 NIH units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• solubility: water: 1 mg/mL, cloudy
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Designed for use in thrombin clotting time tests. Key enzyme that activates factor XIII and converts fibrinogen into fibrin.

Designed for use in thrombin clotting time tests. Serine protease formed in the plasma during the clotting process from prothrombin, calcium, and thromboplasmin. Acts on the soluble plasma protein fibrinogen to convert it to an insoluble fibrin clot. Prepared from homogenous human prothrombin by activation with Factor Xa, Factor Va, and phospholipid. Designed for use in thrombin time tests.

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is determined by comparison with a standard curve prepared using the Bureau of Biologics standard thrombin.

Physical form

Lyophilized from 200 mM NaCl, 50 mM sodium citrate, 0.1% PEG-8000, pH 6.5.

Preparation Note

Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Analysis Note

Complete activation from homogeneous prothrombin by SDS-PAGE

Other Notes

Forsberg, G., et al. 1992. J. Protein Chem.11, 201.
Garcia, J.G. 1992. J. Lab. Clin. Med.120, 513.
Scarborough, R.M., et al. 1992. J. Biol. Chem.267, 13146.
Weigel, L.M., et al. 1992. Infect. Immun.60, 1568.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H315,H319,H334
• Precautionary Statements: P261 - P264 - P280 - P284 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1