567569

Spautin-1

a quinazolin compound that acts as a specific and potent inhibitor of autophagy

• Synonym(s): Spautin-1, Autophagy Inhibitor II, C43, 6-fluoro-N-[4-fluorobenzyl]quinazolin-4-amine, Specific and Potent AUtophagy Inhibitor 1, C43, 6-fluoro-N-[4-fluorobenzyl]quinazolin-4-amine, Specific and Potent AUtophagy Inhibitor 1, Autophagy Inhibitor II
• Empirical Formula (Hill Notation): C₁₅H₁₁F₂N₃
• Molecular Weight: 271.26
• MDL number: MFCD16962292
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥99% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: off-white
• solubility: DMSO: 50 mg/mL, clear, colorless
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

A quinazolin compound that acts as a specific and potent inhibitor of autophagy and promotes Vps34 PI 3-kinase complex degradation by blocking the activity of USP 10 (IC50 = 580 nM) and USP 13 (IC50 = 690 nM) deubiquitinating enzymes. However, it does not affect the lipid kinase activity of Vps34. Also shown to reduce the level of cytoplasmic and nuclear p53. Sensitizes breast cancer cell lines (BCAP37, MCF-7, and BT549) to apoptosis under conditions of nutritional deprivation. Also shown to inhibit etoposide-induced cell death in Bax/Bak double knockout (DKO) cells. Normal cell lines (e.g., MDCK cells) do not undergo autophagy in the presence of Spautin-1.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Lui, J., et al. 2011, Cell.147, 223.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H302,H318
• Precautionary Statements: P264 - P270 - P280 - P301 + P312 - P305 + P351 + P338 - P501
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable