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Key Documents

Safety Information

552095

Sigma-Aldrich

R-Spondin 4, Mouse, Recombinant

Synonym(s):

Cristin 4

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

Assay

≥95% (SDS-PAGE)

Quality Level

100

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

<1.0 EU/μg protein Endotoxin

shipped in

ambient

storage temp.

−20°C

General description

Recombinant, mouse R-Spondin 4 consisting of amino acids 19-228 and expressed in Chinese Hamster Ovary cells. Able to induce activation of β-catenin response in a Luciferase assay using HEK293T human embryonic kidney cells. R-spondin 4 is useful as a positive modulator of Wnt/β signaling.

Biochem/physiol Actions

ED₅₀ = 5-20 ng/ml in the presence of 10 ng/ml rmWnt-3a, as measured by induction of activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells.

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 0.2 µm sterile-filtered PBS, NaCl, EDTA, BSA.

Reconstitution

Reconstitute to 200 µg/ml in PBS.

Other Notes

Hendrickx, M. and L. Leyns. 2008. Develop. Growth Differ.50, 229.
Ishii, Y., et al. 2008. J. Invest. Dermatol.128, 867.
Kim, K.A., et al. 2008. Mol. Biol. Cell19, 2588.
Binnerts, M.E., et al. 2007. Proc. Natl. Acad. Sci. USA104 14700.
Nam, J.S., et al. 2007. Gene Expr. Patterns7, 306.
Pemberton, T.J., et al. 2007. Dev. Dyn.236, 2245.
Bergmann, C., et al. 2006. Am. J. Hum. Genet.79, 1105.
Blaydon, D.C., et al. 2006. Nat. Genet.38, 1245.
Nam, J.S., et al. 2006. J. Biol. Chem.281, 13247.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

552095-25UG:
552095-UG:
552095-25RUG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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