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Safety Information

517312

Sigma-Aldrich

Anti-P-Glycoprotein Mouse mAb (C494)

liquid, clone C494, Calbiochem®

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

C494, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, hamster

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG2a

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Purified mouse monoclonal antibody. Recognizes the ~170 kDa MDR1 isoform of P-glycoprotein.
Recognizes the ~170 kDa MDR1 isoform of P-glycoprotein. Does not cross-react with the MDR3 P-glycoprotein.
This Anti-P-Glycoprotein Mouse mAb (C494) is validated for use in Paraffin Sections, FC, Frozen Sections, Immunoblotting, ICC, IP for the detection of P-Glycoprotein.

Immunogen

Epitope: gene-specific internal cellular epitope present only on the MDR1 isoform of P-glycoprotein
Hamster and Human
SDS-solubilized plasma membranes of a multidrug resistant (MDR) Chinese hamster ovary (CHO) cell line and a human leukemic cell line

Application

Paraffin Sections (5-10 µg/ml, see comments)

Flow Cytometry (see comments)

Frozen Sections (5-10 µg/ml, see comments)

Immunoblotting (see comments)

Immunocytochemistry (see application references)

Immunoprecipitation (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, 1% BSA.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Cell lines or frozen tissue, such as human liver or colon.

Other Notes

Does not cross-react with MDR3 P-glycoprotein. Also reported to work for flow cytometry, immunoblotting, and immunoprecipitation. For immunocytochemistry, frozen or paraffin-embedded material may be used. Permeabilization of the cell membrane is required to expose the epitope recognized by this antibody. Variables associated with assay conditions will dictate the proper working dilutions.
Rao, V.V., et al. 1994. Cancer Res.54, 1536.
Toth, K., et al. 1992. Am. J. Pathol.140, 1009.
Georges, E., et al. 1990. Proc. Natl. Acad. Sci. USA87, 152.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

517312-UL:
517312-500UL:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Regino Mercado-Lubo et al.
Nature communications, 7, 12225-12225 (2016-07-28)
Salmonella enterica serotype Typhimurium is a food-borne pathogen that also selectively grows in tumours and functionally decreases P-glycoprotein (P-gp), a multidrug resistance transporter. Here we report that the Salmonella type III secretion effector, SipA, is responsible for P-gp modulation through

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