Skip to Content
Merck
All Photos(1)

Key Documents

Safety Information

481409

Sigma-Aldrich

PTEN, His-Tag® and S-Tag, Human Recombinant, S. frugiperda

Synonym(s):

Phosphatase and Tensin Homolog Deleted on Chromosome 10

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352202
NACRES:
NA.42

Assay

≥80% (SDS-PAGE)

Quality Level

100

form

liquid

specific activity

≥75 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

shipped in

wet ice

storage temp.

−70°C

General description

Full-length, recombinant, human PTEN (GenBank target symbol = PTEN, ACC No. BC_005821) fused at N-terminus to a His•Tag and S•Tag sequences and expressed in S. frugiperda insect cells. PTEN is a tumor suppressor that is frequently found mutated in human cancers. It serves as a lipid phosphatase and negative regulator of the PI-3K/Akt pathway. Absence of functional PTEN results in constitutive activation of Akt and mTOR kinases.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme required to liberate 1 nmol phosphate from PIP₃ per min at 37°C.

Physical form

In 150 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 1 mM EDTA, 50% glycerol, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Harrington, L.S., et al. 2005. Trends Biochem. Sci.30, 35.
Osaki, M., et al. 2004. Apoptosis9, 667.
Lennon, G., et al. 1996. Genomics33, 151.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

481409-10UG:
481409-2.5UG:
481409-UG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service