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Key Documents

Safety Information

437620

Sigma-Aldrich

Lipopolysaccharide, E. coli J5

Mutant of E. coli O111: B4. Stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes.

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About This Item

UNSPSC Code:
12352201
NACRES:
NA.77

Quality Level

100

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

<2.5% Protein
5.0-10.0% 2-keto-3-deoxyoctonate (KDO)

solubility

water: 1 mg/mL

anion traces

phosphate (PO43-): 6.0-15.0%

shipped in

ambient

storage temp.

2-8°C

General description

Mutant of E. coli 0111:B4. Stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes. Highly purified preparation.
Mutant of E. coli O111: B4. Stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Inducible nitric oxide synthase
Product does not compete with ATP.
Reversible: no

Warning

Toxicity: Harmful (C)

Preparation Note

LPS will form a suspension in H₂O; slight warming and vortexing may be required to achieve complete dispersion in H₂O. Addition of 0.5% triethylamine will also help with dispersion; triethylamine is very basic and can be neutralized with Trix-HCl to avoid hydrolysis of the fatty acid chains.

Reconstitution

Following reconstitution refrigerate (4°C). Stock solutions are stable for up to 1 year at 4°C.

Other Notes

Hattori, S., et al. 1995. Biochem. Mol. Biol. Int.35, 177.
Mayeux, P.R., et al. 1995. Biochem. Pharmacol.49, 115.
Norimatsu, M., et al. 1995. Infect. Immun.63, 1122.
Feinstein, D.L., et al. 1993. J. Neurochem.60, 1945.
Zhang, Y.H., et al. 1993. Infect. Immun.61, 5044.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Skull and crossbones
GHS06

Signal Word

Danger

Hazard Statements

H300

Hazard Classifications

Acute Tox. 2 Oral

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

437620-MG:
437620-5MG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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