420127

Jasplakinolide

from Jaspis johnstoni, ≥90% (HPLC), liquid, Actin polymerization promotor, Calbiochem

• Synonym(s): InSolution Jasplakinolide, Jaspis johnstoni
• Empirical Formula (Hill Notation): C₃₆H₄₅BrN₄O₆
• Molecular Weight: 709.67
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Product Name: Jasplakinolide, Jaspis johnstoni, InSolution, ≥90%, 1 mM in DMSO, mammablian cell mitosis inhibitor, potent inducer of actin polymerization and stabilization
• Quality Level: 100
• Assay: ≥90% (HPLC)
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic); protect from light
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

A cell-permeable F-actin probe. This macrocyclic cyclodepsipeptide, isolated from the marine sponge Jaspis johnstoni, is a potent inducer of actin polymerization and stabilization in vitro. It competes with Phalloidin (Cat. No. 516640) for actin binding (K_d = 15 nM) and enhances apoptosis induced by interleukin 2 (IL-2) deprivation. Also displays fungicidal, insecticidal, and anticancer properties.

Biochem/physiol Actions

Cell permeable: no

Kd = 15 nM for actin binding

Primary Target
F-actin

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Harmful (C)

Physical form

A 1 mM (50 µg/71 µl) solution of Jasplakinolide, Jaspis johnstoni (Cat. No. 420107) in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Rosado, J.A., et al. 2000. J. Biol. Chem.275, 7527.
Posey, S.C., and Bierer, B.E. 1999. J. Biol. Chem.274, 4259.
Senderowicz, A.M., et al. 1995. J. Natl. Cancer Inst.87, 46.
Bubb, M.R., et al. 1994. J. Biol. Chem.269, 14869.
Scott, V.R., et al. 1988. Antimicrob. Agents Chemother.32, 1154.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• Flash Point(C): 87 °C - closed cup - (Dimethylsulfoxide)