410957

3-Isobutyl-1-methylxanthine

A cell-permeable, non-specific inhibitor of cAMP and cGMP phosphodiesterases (IC₅₀ = 2-50 µM).

• Synonym(s): 3-Isobutyl-1-methylxanthine, IBMX
• Empirical Formula (Hill Notation): C₁₀H₁₄N₄O₂
• CAS Number: 28822-58-4
• Molecular Weight: 222.24
• MDL number: MFCD00005584
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: ≥98% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• solubility: ethanol: 10 mg/mL; DMSO: 100 mM; methanol: 50 mg/mL
• shipped in: ambient
• storage temp.: −20°C
• InChI: 1S/C10H14N4O2/c1-6(2)4-14-8-7(11-5-12-8)9(15)13(3)10(14)16/h5-6H,4H2,1-3H3,(H,11,12)
• InChI key: APIXJSLKIYYUKG-UHFFFAOYSA-N

Description

General description

A cell-permeable, non-specific inhibitor of cAMP and cGMP phosphodiesterases (IC₅₀ = 2-50 µM). Also acts as an adenosine receptor antagonist. Reported to inhibit TNF-α expression in adipocyte precursor cells.

A non-specific, cell-permeable inhibitor of cAMP and cGMP phosphodiesterases (IC₅₀ = 2-50 µM). Also acts as an adenosine receptor antagonist. Reported to inhibit TNFα expression in adipocyte precursor cells.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
cAMP and cGMP phosphodiesterases

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 2-50 µM against cAMP and cGMP phosphodiesterases

Warning

Toxicity: Standard Handling (A)

Preparation Note

Heating may be required to achieve complete solubilization.

Reconstitution

Following reconstitution in ethanol or methanol, refrigerate (4°C). Following reconstitution in DMSO, aliquot and freeze (-20°C). Ethanol/methanol and DMSO stock solutions are stable for up to 6 months at 4°C or -20°C, respectively.

Other Notes

Hube, F., et al. 1999. Horm. Metab. Res.31, 359.
Morgan, A.J., et al. 1993. Biochem. Pharmacol.45, 2373.
Scamps, F., et al. 1993. Eur. J. Pharmacol. 244, 119.
Turner, N.C., et al. 1993. Br. J. Pharmacol. 108, 876.
Tamura, T., et al. 1991. J. Gen. Physiol. 98, 95.
Beavo, J.A., and Reifsnyder, D.H. 1990. Trends Pharmacol. Sci.11, 150.
Russell, T.R. 1979. Proc. Natl. Acad. Sci. USA76, 4451.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable