407303

γ-Interferon, Mouse, Recombinant, E. coli

• Synonym(s): IFNG, Type II IFN
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• description: Merck USA index - 14, 4992
• Quality Level: 100
• Assay: ≥95% (SDS-PAGE)
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• solubility: water: soluble
• Protein ID accession no.: ABN80441.1
• UniProt accession no.: P01580
• shipped in: wet ice
• storage temp.: −70°C
• Gene Information: mouse ... Ifng(15978)

Description

General description

Note: 1 KU = 1000 units.

Research area: Immunology and Cytokines

Recombinant, mouse γ-interferon, consisting of 134 amino acids, expressed in E. coli as a single, non-glycosylated polypeptide.

Application

γ-Interferon has been used to assess the response of wild-type and cytosolic phospholipase A2 (cPLA2)-deficient cells to IFN-mediated signals. It has also been used to study the signaling pathway associated with IFN-γ and to explore the potential role of β-Arrestin1 in the dephosphorylation of signal transducer and activator of transcription 1 (STAT1).

Biochem/physiol Actions

Interferon-gamma (IFN-γ), a proinflammatory cytokine, exhibits a significant impact on innate and adaptive immunity in terms of combatting intracellular infections and facilitating tumor control. It has a significant impact on various immunological pathways. IFN-γ influences the following biological processes:

Antigen Processing and Presentation: IFN-γ enhances the expression of molecules involved in antigen presentation. This helps in the efficient activation of T cells, leading to the initiation of adaptive immunity.

Reactive Oxygen Species (ROS): IFN-γ stimulates the production of ROS by promoting the assembly of NADPH oxidase subunits. These ROS possess strong microbicidal effects, aiding in the defense against invading pathogens.

Autophagy: IFN-γ induces the expression of proteins involved in autophagy, facilitating the clearance of intracellular pathogens and contributing to immune defense.

Apoptosis: IFN-γ triggers apoptotic cell death by increasing the expression of molecules associated with the apoptotic pathway.

Cell Migration and Leukocyte Traffic: IFN-γ promotes the expression of chemokines and adhesion molecules, facilitating immune cell migration and contributing to the initiation of inflammatory responses.

Antiviral Mediators: By inducing the expression of various antiviral proteins, IFN-γ counteracts viral infections at multiple stages of the viral life cycle.

Warning

Toxicity: Standard Handling (A)

Physical form

In 10 mM PBS, pH 7.4.

Preparation Note

Dilute only in buffers containing protein such as 0.1% BSA.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Zantl, N., et al. 1998. Infect. Immun. 66, 2300.
Familletti, P.C., et al. 1981. Methods Enzymol. 78, 387.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable