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Key Documents

Safety Information

407256

Sigma-Aldrich

IGF-II, CF, Mouse, Recombinant

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.75

Assay

≥97% (SDS-PAGE)

Quality Level

100

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

<1.0 EU/μg protein Endotoxin

shipped in

ambient

storage temp.

−20°C

General description

Recombinant, mouse insulin-like growth factor II (IGF-II) consisting of amino acids 25-91 and expressed in E. coli. Mouse IGF-II is synthesized as a 180 amino acid preproprecursor. Mature IGF-II is 91% and 97% amino acid identical to human and rat IGF-II, respectively.

Biochem/physiol Actions

ED₅₀ = 2-10 ng/ml as measured in a serum-free cell proliferation assay using MCF-7 human breast adenocarcinoma cells.

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 0.2 µm sterile-filtered Acetonitrile, TFA.

Reconstitution

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 100 µg/ml in sterile PBS.

Other Notes

Ostrovsky, O., et al. 2009. Mol. Biol. Cell20, 1855.
Chao, W. & P. A. DAmore, 2008. Cytokine Growth Factor Rev.19, 111.
Belfiore, A., 2007. Curr. Pharm. Des.13, 671.
Cornish, J., et al. 2007. Am. J. Physiol. Endocrinol. Metab.292,E117.
Merrick, D., et al. 2007. BMC Dev. Biol.7, 65.
Pavelic, J., et al. 2007. Indian J. Med. Res.125, 511.
VarelaNieto, I., et al. 2007. Curr. Pharm. Des.13,687.
Denley, A. et al. 2005. Cytokine Growth Factor Re,v.16, 421.
LeRoith, D. & C.T. Roberts Jr., 2003. Cancer Lett.195,127.
Buchanan, C.M., et al. 2001. Biochem. J.360, 431.
Werner, H. & D. LeRoith, 2000. Cell. Mol. Life Sci.57, 932.
Goya, L., et al. 1999. J. Biol. Chem.274 24633.
Daughaday, W.H., et al. 1993. Proc. Natl. Acad. Sci. USA90, 5823.
Holthuizen, P.E., et al. 1993. Regul. Pept.48, 77.
McCarthy, T.L., et al. 1992. Endocrinology130, 1303.
Zindy, F., et al. 1992. J. Hepatol.14, 30.
Rotwein, P. & L.J. Hall, 1990. DNA Cell Biol.10,725.
Rotwein, P., et al. 1988. Proc. Natl. Acad. Sci. USA85, 265.
Stempien, M.M., et al. 1986. DNA5, 357.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

4.1B - Flammable solid hazardous materials

WGK

WGK 2

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

407256-UG:
407256-50RUG:
407256-50UG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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