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Safety Information

371208

Sigma-Aldrich

Dkk-1, Rat, Recombinant

Dkk-1, Rat, Recombinant, consists of amino acids 23-270, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells.

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.27

recombinant

expressed in mouse NSO cells

Quality Level

100

Assay

≥95% (SDS-PAGE)

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤1.0 EU/μg protein Endotoxin

shipped in

ambient

storage temp.

−20°C

General description

Dkk-1, Rat, Recombinant, consists of amino acids 23-270, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells.
Recombinant, rat Dickkopf related protein-1 (Dkk-1), consisting of amino acids 23-270, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells. The balance between Wnt signaling and Dkk-1 inhibition is essential for bone formation and homeostasis.

Biochem/physiol Actions

ND₅₀ = 0.1-0.5 µg/ml as measured by inhibition of Wnt-3a-induced (10 ng/ml rmWnt-3a) alkaline phosphatase production by proliferation of MC3T3-E1 mouse osteoblastic cells.

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 0.2 µm filtered PBS, BSA.

Reconstitution

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.

Other Notes

Li, J. et al. 2006. Bone36, 754.
Morvan, F. et al. 2006. J. Bone Miner. Res.21, 934.
Niehrs, C. 2006. Oncogene25, 7469.
Verani, R. et al. 2006. J. Neurochem.101, 242.
Cappuccio, I. et al. 2005. J. Neurosci.25, 2647.
Kemp, C. et al. 2005. Dev. Dyn.233, 1064.
Adamska, M. et al. 2004. Dev. Biol.272, 134.
Bullock, C.M. et al. 2004. Mol. Pharmacol.65, 582.
Mao, B. et al. 2002. Nature417, 664.
Mao, B. et al. 2001. Nature411, 321.
Krupnik, V.E., et al. 1999. Gene238, 301.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

371208-10RUG:
371208-10UG:
371208-UG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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