33-017

PI 3-Kinase HTRF^® Assay; 1920 wells

The PI 3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format. This pack size has a screening capacity of 5 plates.

• Synonym(s): Kinase activity assay
• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

Properties

• Quality Level: 100
• species reactivity: mouse, human
• manufacturer/tradename: HTRF^®; Upstate^®
• technique(s): activity assay: suitable (kinase)
• NCBI accession no.: NM_006218.2
• UniProt accession no.: P42336
• detection method: fluorometric (HTRF)
• shipped in: dry ice
• Gene Information: human ... PIK3CA(5290)

Description

General description

The PI3-Kinase HTRF Assay is a high-performance assay kit that provides a universal method for assaying all Class I PI3-Kinases in a homogeneous 384-well format.

Application

PI 3-Kinase HTRF^® Assay; 1920 wells have been used to measure the activity of phosphoinositide-3-kinases (PI3Ks). It has also been used in a biochemical assay to measure the potency and selectivity of LL-00071210 (a selective PI3Kδ inhibitor).

Biochem/physiol Actions

The PI 3-Kinases catalyze the phosphorylation of the three positions of phosphatidylinositols. The Class I enzymes convert phosphatidylinositol 4,5-bisphosphate (PIP2) to phosphatidylinositol 3,4,5-trisphosphate (PIP3) in the presence of ATP and Mg²⁺.

Features and Benefits

• Large Stokes shift and time-resolved elements of technology help to reduce compound interference, minimizing concern of false negatives
• Picomole sensitivity of the HTRF assay platform enables the integrity of enzyme kinetics to be retained, ensuring the most physiologically relevant assay parameters.
• Compatible with all Class I phosphoinositide 3-kinase (PI3K) isoforms: enables relative data comparisons and eliminates the need for multiple assay types.
• Reliable, proven assay—based on the technology used in the Millipore, Upstate^® KinaseProfiler Service.
• Z` factor routinely exceeds 0.8, ensuring consistent, reliable data.

Packaging

Kit capacity: 1920 wells

Components

4X Reaction Buffer

PIP2 (Substrate; 1mM stock)

Stop Reagent A

Stop Reagent B

DM A Detection Reagent

DM B Detection Reagent

DM C Detection Reagent

Legal Information

HTRF is a registered trademark of Cis Bio International

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319
• Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2