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Safety Information

324390

Sigma-Aldrich

Anti-Dopamine D₁ Receptor Rabbit pAb

lyophilized, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized

does not contain

preservative

species reactivity

human, mouse, rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... DRD1(1812)
rat ... Drd1(24316)

General description

Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~50 kDa dopamine D1 receptor protein.
Recognizes the dopamine D1 receptor in rat brain. Does not cross-react with other dopamine receptors.
This Anti-Dopamine D₁ Receptor Antibody is validated for use in ELISA, WB, Frozen Sections, Free-Floating Sections, Paraffin Sections for the detection of Dopamine D₁ Receptor.

Immunogen

Human
a synthetic peptide (MDGTGLVVERDFSC) corresponding to amino acids 9-21 of human D₁dopamine receptor

Application

ELISA (see comments)

Immunoblotting (1:1000)

Frozen Sections (1:5000)

Free-Floating Sections (1:5000)

Paraffin Sections (not recommended)

Warning

Toxicity: Standard Handling (A)

Physical form

Undiluted serum.

Reconstitution

Reconstitute the antibody with 100 µl PBS containing 1% BSA. For further dilution of the antibody use a similar buffer formulation. Following reconstitution, refrigerate (4°C).

Analysis Note

Positive Control
Rat brain

Other Notes

Mignini, F., et al. 2000. J. Autonom. Pharmacol.20, 325.
This antibody has also been reported to work in ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat′s milk. For frozen sections a 1:5000 dilution of the reconstituted serum demonstrates specific staining of rat brain sections including the medial septum, nucleus accumbens, dentate gyrus, globus pallidus, medial forebrain bundle, cortex regions 1-3, substantia nigra reticulata, and the ventral tegmental area. Strong nuclear staining of oligodendrocytes was also observed. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

324390-UL:
324390-100UL:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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