240896

Cystatin C, Human Urine

A low molecular weight non-glycosylated basic protein of the cystatin superfamily of cysteine protease inhibitors that it is freely filtered by the glomerular membrane.

• Synonym(s): Post γ-globulin
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: ≥95% (SDS-PAGE)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• solubility: 0.1 M sodium acetate, pH 4.5: soluble
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

A low molecular weight non-glycosylated basic protein of the cystatin superfamily of cysteine protease inhibitors that is freely filtered by the glomerular membrane. Serum levels of cystatin C can be used as a specific and sensitive marker of glomerular filtration rate. Protects organisms against undesirable proteolysis by endogenous or exogenous enzymes. Rapidly and tightly inhibits papain (K_i = 0.26 nM) and cathepsin B (K_i = 4.4 nM). Thought to be linked to many disease processes including Alzheimer′s Disease, hereditary cerebral hemorrhage and lung cancer.

A low molecular weight non-glycosylated basic protein of the cystatin superfamily of cysteine protease inhibitors that it is freely filtered by the glomerular membrane. Serum levels of cystatin C can be used as a specific and sensitive marker of glomerular filtration rate. Protects organisms against undesirable proteolysis by endogenous or exogenous enzymes. Thought to be linked to many disease processes including Alzheimer’s disease, hereditary cerebral hemorrhage, and lung cancer.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Cysteine protease

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 20 mM NH₄HCO₃.

Preparation Note

Prepared from the urine of patients with chronic renal tubular proteinuria that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following reconstitution, store in the refrigerator at 4°C. Stock solutions are stable for up to 2 month when stored at -20°C.

Other Notes

Hall, A., et al. 1998. Biochemistry37, 4071.
Peloille, S., et al. 1997. Eur. J. Biochem. 244, 140.
Cimerman, N., et al. 1996. Biol. Chem. Hoppe Seyler 377, 19.
Tenstad, O., et al. 1996. Scand. J. Clin. Lab. Invest.56, 409.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable