2160
Autofluorescence Eliminator Reagent
Autofluorescence Eliminator Reagent for IHC.
Synonym(s):
fluorescence IHC counter stain
About This Item
Recommended Products
form
liquid
packaging
pkg of 50 mL
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
shipped in
ambient
General description
Application
PROTOCOL:
1.After immunofluorescence histochemistry, the sections are immersed in PBS for 5 min.·
2.Sections are immersed in 70% ethanol for 5 min.·
3.Sections are immersed in Autofluorescence Eliminator Reagent for 5 min.
4.Sections are immersed in three changes of 70% ethanol for 1 min. each time.·
5.Sections are mounted using an antifading solution (e.g. DABCO, non-xylene-based).
Note: Reagent will block all fluorescence if incubation is too long; length of incubation time and number of 70% ethanol washes can be altered and optimized for various tissues and fluorescent antibody probes. In some instances the reagent can be applied prior to immunofluorescence, however in most cases better results are obtained when used as we direct above (use after immunofluorescence procedures are completed).
Optimal time of incubation with the Autofluorescence Eliminator Reagent should be determined by the end user for each sample.
Absorption maximum: 595-605
Physical form
Storage and Stability
Legal Information
Disclaimer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Muta. 2 - STOT SE 2
Target Organs
Eyes,Central nervous system
Storage Class Code
3 - Flammable liquids
WGK
WGK 2
Flash Point(F)
60.8 - 68.0 °F
Flash Point(C)
16 - 20 °C
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
FSL
Group 4: Flammable liquids
Alcohols
Hazardous rank II
ISHL Indicated Name
Substances Subject to be Indicated Names
ISHL Notified Names
Substances Subject to be Notified Names
JAN Code
2160:
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