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17-295

Sigma-Aldrich

Chromatin Immunoprecipitation (ChIP) Assay Kit

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using inexpensive protein A agarose beads.

Synonym(s):
Agarose ChIP Kit
eCl@ss:
32161000
NACRES:
NA.41

Quality Level

manufacturer/tradename

EZ-ChIP

technique(s)

immunoprecipitation (IP): suitable

shipped in

wet ice

Related Categories

General description

For use to enrich for specific regions of chromatin that are immunoprecipitated with a specific antibody for a chromatin protein. Works with all eukaryotic cell types. Detection of the gene or promoter of interest in immunoprecipitated chromatin must be empirically determined by the researcher. Quantitative PCR detection is recommended.

Packaging

Kit capacity: 22 Assays

Components

Protein A agarose/Salmon Sperm DNA (Cat.# 16-157)

All necessary buffers

Quality

Routinely evaluated using anti-acetyl Histone H4 polyclonal antibody (06-866) to immunoprecipitate acetylated histone H4. Subsequent immunoblot analysis was done using the same antibody.

Physical form

Contains all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Certificate of Analysis

Certificate of Origin

Adjacent sequence controls the response polarity of nitric oxide-sensitive Sp factor binding sites
Zhang, J., et al
The Journal of Biological Chemistry, 278, 29192-29200 (2003)
Demethylase activity is directed by histone acetylation
Cervoni, N. and Szyf, M.
The Journal of Biological Chemistry, 276, 40778-40787 (2001)
A functionally conserved boundary element from the mouse HoxD locus requires GAGA factor in Drosophila.
Dasari Vasanthi,Mamidi Anant,Surabhi Srivastava,Rakesh K Mishra
Development null
D Pannell et al.
The EMBO journal, 19(21), 5884-5894 (2000-11-04)
Retrovirus vectors are de novo methylated and transcriptionally silent in mammalian stem cells. Here, we identify epigenetic modifications that mark retrovirus-silenced transgenes. We show that murine stem cell virus (MSCV) and human immunodeficiency virus type 1 (HIV-1) vectors dominantly silence
Orexin signaling mediates the antidepressant-like effect of calorie restriction.
Lutter, M; Krishnan, V; Russo, SJ; Jung, S; McClung, CA; Nestler, EJ
The Journal of Neuroscience null

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