Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

Safety Information

17-218

Sigma-Aldrich

Ras Activation Assay Kit

Synonym(s):

Ras Activity Assay

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84

manufacturer/tradename

Upstate®

Quality Level

100

technique(s)

activity assay: suitable

NCBI accession no.

NM_001085049.1
NM_012219.3

UniProt accession no.

O14807

shipped in

dry ice

Gene Information

human ... MRAS(22808)

General description

Muscle RAS oncogene homolog (MRAS) plays a key role in cardiovascular diseases. The MRAS gene codes for a member of the ras superfamily of GTP-binding proteins. MRAS is found in all tissues, particularly in the cardiovascular system. It is involved in signal transduction, including cell growth and differentiation.

Packaging

Kit capacity: 30 assays

Components

Ras Assay Reagent (Raf-1 RBD, agarose) (Cat.# 14-278)

Anti-Ras, clone RAS10 (Cat.# 05-516)

100X GTPγS, 10mM (Cat.# 20-176)

100X GDP, 100mM (Cat.# 20-177)

Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)

Quality

This kit precipitated Ras-GTP from HeLa cell lysates that had been loaded with GTPγS. The precipitated Ras-GTP was detected by western immunoblot using 1μg/ml anti-Ras, clone RAS10

Linkage

Replaces: SGT435

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

CorrosionEnvironment
GHS05,GHS09

Signal Word

Danger

Hazard Statements

H318,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1

Storage Class Code

10 - Combustible liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

17-218:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Fu-Ming Tsai et al.
Cellular signalling, 18(3), 349-358 (2005-07-12)
The retinoid-inducible gene 1 (RIG1) protein is a retinoid-inducible growth regulator. Previous studies have shown that the RIG1 protein inhibits the signaling pathways of Ras/mitogen-activated protein kinases. However, neither the mode of action nor the site of inhibition of RIG1
Michael R Bennett et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 23(18), 7207-7217 (2003-08-09)
Neurofibromatosis type 1 (NF1) patients are predisposed to learning disabilities, macrocephaly, and brain tumors as well as abnormalities on magnetic resonance imaging that are postulated to result from abnormal myelination. Here we show that Nf1+/- spinal cords in adult mice
Richard J Calvert et al.
Analytical biochemistry, 343(2), 283-292 (2005-07-16)
Accurate measurement of activity of wild-type K-ras protein is important due to its tumor suppressor action in tissues such as lung. A published method by Taylor and co-workers uses plasmid-containing Escherichia coli cells to produce a glutathione-S-transferase/raf-1 ras binding domain
Fu-Ming Tsai et al.
Cellular signalling, 19(5), 989-999 (2007-01-02)
Retinoid-inducible gene 1 encodes RIG1 is a growth regulator, which inhibits the pathways of the RAS/mitogen-activated protein kinases by suppressing the activation of RAS. Confocal microscopic analysis demonstrated that RIG1 is localized in the endoplasmic reticulum (ER) and Golgi apparatus
Shin-ichiro Miura et al.
Biochemical and biophysical research communications, 306(4), 924-929 (2003-06-25)
Since we recently reported that high density lipoprotein, which contains the bioactive lipid sphingosine-1-phosphate (S1P) [Arterioscler. Thromb. Vasc. Biol. 23 (2003) 802], induced human coronary artery endothelial cell (HCEC) tube formation mediated by a Ras/Raf/ERK (extracellular signal-activated kinase) pathway, we
View All Related Papers

Articles

Probiotics and Human Health

Today, diverse studies report the benefits of probiotics, such as inhibitory effects on pathogens, aid in the management or prevention of chronic intestinal inflammatory diseases or atopic syndromes, and support to the immune system. Potential beneficial applications abound, researchers continue to evaluate the effictiveness and clarify the mechanisms of action of probiotics.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service