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Safety Information

14-837

Sigma-Aldrich

NAP1 Protein, active, 300 µg

Active N-terminal His6-tagged Drosophila NAP1 expressed in E. coli.

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.32

Quality Level

100

mol wt

Mw 43 kDa

manufacturer/tradename

Upstate®

UniProt accession no.

Q9W1G7

shipped in

dry ice

Gene Information

Drosophila ... NAP1(37798)

General description

N-terminal 6×His-tagged Drosophila NAP1 expressed in E. coli.
Nucleosome assembly proteins (NAP) are involved in moving histones into the nucleus, nucleosome assembly and chromatin fluidity. They affect the transcription of many genes.

Application

Lot # R0803D0002: 12.5 µg NAP1 has been successfully used for 1 chromatin assembly reaction with Chromatin Assembly Kit (Millipore Corp, Catalog# 17-410).

Quality

Activity is routinely tested with Chromatin Assembly Kit (Millipore Corp, Catalog# 17-410).

Physical form

10 mM K-HEPES pH 7.6, 10 mM KCl, 50 mM NaCl, 0.1 mM EDTA, 10%(v/v) glycerol, 0.01% NP-40, 0.2 mM PMSF, 1mM DTT. Frozen solution.
Format: Purified

Storage and Stability

Stable for 6 months at -80°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Other Notes

Specific Activity: Varies with lot
Varies with individual lot. Lot R0803D0002 has purity 90% by SDS-PAGE and Coomassie blue staining

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

14-837:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Roberto Fabian et al.
Molecules (Basel, Switzerland), 26(16) (2021-08-28)
We report data from single molecule studies on the interaction between single DNA molecules and core histones using custom-designed horizontal magnetic tweezers. The DNA-core histone complexes were formed using λ-DNA tethers, core histones, and NAP1 and were exposed to forces

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