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14-440-M

Sigma-Aldrich

PKA Protein, Recombinant, 10 µg

Recombinant human full length PKA, catalytic subunit type alpha, untagged, for use in Kinase Assays.

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About This Item

UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.41

biological source

human

Quality Level

100

recombinant

expressed in E. coli
expressed in BL21 DE3 cells

product line

Upstate®

specific activity

(For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)

mol wt

Mw 40.7 kDa

purified by

chromatography

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

accession no.

 (FUNCTION: SwissProt: P17612 # Phosphorylates a large number of substrates in the cytoplasm and the nucleus. )
NM_002730.3

NCBI accession no.

NM_002730.3
NM_207518.1

UniProt accession no.

P17612

General description

Recombinant human full length PKA, catalytic subunit type alpha, untagged

Biochem/physiol Actions

Protein Target: PKA
Target Sub-Family: AGC

Packaging

Also available in 10 μg size and in bulk
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-440M when ordering the 250 µg size.

Quality

routinely evaluated by phosphorylation of Kemptide substrate in vitro

Target description

PKA

Linkage

Replaces: 14-114

Physical form

Phosphocellulose P11 chromatography

Storage and Stability

1 year at -70°C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Prokaryotic expression of catalytic subunit of adenosine cyclic monophosphate-dependent protein kinase.
Yonemoto, W M, et al.
Test, 200, 581-596 (1991)
Dana Yoo et al.
The Journal of biological chemistry, 278(25), 23066-23075 (2003-04-10)
The Kir1.1 (ROMK) subtypes of inward rectifier K+ channels mediate potassium secretion and regulate sodium chloride reabsorption in the kidney. The density of ROMK channels on the cortical collecting duct apical membrane is exquisitely regulated in concert with physiological demands.

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