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LiChrospher® 100 RP-8 (5 µm) HPLC Columns

HPLC sorbent

Synonym(s):

TM=["LiChrospher"] C8 sorbent for HPLC

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52

product name

LiChrospher® 100 RP-8 (5 µm),

material

stainless steel column

Agency

suitable for USP L7

product line

LiChrospher®

feature

endcapped: no

extent of labeling

12.5% Carbon loading

parameter

60 °C max. temp.

technique(s)

HPLC: suitable
mass spectrometry (MS): suitable (Low - Medium)

surface area

350 m2/g

matrix

fully porous particle

matrix active group

C8 bonding phase

particle size

5 μm

pore size

100 Å pore size

operating pH range

2-7.5

bulk density

200‑400 kg/m3

separation technique

reversed phase

storage temp.

no temp limit

General description

LiChrospher® RP-8 is a reliable and versatile traditionally produced spherical silica gel carrier with C-8 reversed phase properties. It is well suited for the separation of neutral, acidic and weak basic compounds.

Application


  • Development and validation of a HPLC-UV method for the determination in didanosine tablets.: This study presents a validated HPLC-UV method optimized using LiChrospher® 100 RP-8 (5 µm) for analyzing didanosine in tablet formulations, offering a reliable tool for quality control in pharmaceutical environments (de Oliveira et al., 2005).

  • Determination of steroid hormones in oral contraceptives by high-performance liquid chromatography.: Utilizes LiChrospher® 100 RP-8 (5 µm) to effectively separate and quantify steroid hormones in oral contraceptive pills, demonstrating the column′s applicability in complex pharmaceutical analyses (Inês et al., 2002).

  • Determination of fluoroquinolone residues in animal tissues by liquid chromatography.: Explores the use of LiChrospher® 100 RP-8 (5 µm) for the sensitive detection of fluoroquinolone residues in animal tissues, highlighting its potential for food safety and veterinary medicine applications (Posyniak et al., 1999).

  • Rapid and simple determination of oxytetracycline in chicken products.: Demonstrates the column′s efficiency in rapidly quantifying oxytetracycline residues in chicken, ensuring compliance with food safety standards (Furusawa, 1999).

  • Crocheted ETFE-reactor for on-line post-column photoderivatization of diclofenac in high-performance liquid chromatography.: Describes an innovative application involving LiChrospher® 100 RP-8 (5 µm) in a post-column reactor setup to enhance the detection sensitivity of diclofenac, underlining the column′s versatility in advanced analytical techniques (Kuhlmann et al., 1997).


Analysis Note

Application test: passes test
Pressure: ≤ 45 bar
Theoretical Plates (N/m) (Toluene): ≥ 60000
Capacity factor (Toluene): ≥ 0.7
stability testing (performance): passes test
separation factor (PTH-Lysine/PHB-Propyl-Ester, selectivity test): 1.20 - 1.55
separation factor (Naphthalene/Digitoxigenin, selectivity test): 3.5 - 4.7
separation factor (PTH-Lysine/Digitoxigenin, selsctivity test): 1.70 - 2.25
Particle Size (d10): 4.8 - 5.5 µm
Particle Size (d50): 6.5 - 7.5 µm
Particle Size (d90): 9.0 - 10.5 µm

Legal Information

LiChrospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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