Skip to Content
Merck
All Photos(1)

Key Documents

1.05642

Supelco

HPTLC plates, Silica gel 60 F₂₅₄

pkg of 50 plates, plate L × W 20 cm × 10 cm, glass support

Synonym(s):

HPTLC Silica Gel Plates

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41115711
NACRES:
NB.21

material

glass support
silica gel 60 matrix

Quality Level

feature

binder Organic Polymer
fluorescent indicator

packaging

pkg of 50 plates

technique(s)

thin layer chromatography (TLC): suitable

layer thickness

200 μm

plate L × W

20 cm × 10 cm

particle size

5-6 μm

pore size

60 Å medium pore diameter

storage temp.

2-30°C

General description

50 Glass plates 20 x 10 cm
HPTLC plates deliver fast and quantitative analysis of complex samples for manual or automated use. Our HPTLC silica plates work three times faster than conventional TLC plates – and they are more sensitive. The particle sizes range in HPTLC is between 4-8 µm, which creates a smoother surface and a higher separation power than TLC plates.

Application

Glass HPTLC Silica gel 60 F₂₅₄ plates. Silica gel HPTLC plates size 20 x 10 cm, 50 sheets.

Linkage

Replaces: 5642-6; 5642

Analysis Note

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Colour test
hRf-values
- bleu vif organol, colour test, lipophile:
17 - 22
- Ceres Black G, colour test, lipophile: 38 - 43
- ceres Violet brn, colour test, lipophile: 58 - 64
separation number
- colourtest, lipophile:
≥ 10.0
B) Steroid test
hRf-values
- hydrocortisone, steroid test:
23 - 29
- Reichstein S, steroid test: 36 - 45
- methyltestosterone, steroid test: 43 - 52
separation number
- steroid test:
≥ 10.5
Typical value determined on a conditioned plate
Eluent A) Toluene (45% rel.humidity)
Eluent B) Ethyl acetate/Toluene (95+5 v/v; 20% rel.humidity)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

In this article we present several HPTLC applications and analytical standards for ginsenosides.

HPTLC is an efficient method to determine Steviol Glycosides content in Stevia using our HPTLC plates and reference materials, including a new introduced Stevia extract reference material.

Protocols

In the present study we demonstrate compliance of silica gel 60 F254 HPTLC plates with USP requirements, and how HPTLC plates are suitable for the Sildenafil citrate-Limit of Imidazole test.

Powdered black pepper was chosen as example for dietary supplements, being a source for pierine. It is tested according to the current United States Pharmacopeia (USP) guidelines using HPTLC and HPLC.

Effective analysis of passion flower by HPTLC fingerprinting, in conjunction with a comprehensive range of related reference materials, enables fast and efficient identification of this plant material.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service