1.05449

Wort broth (base)

GranuCult^®, suitable for microbiology, for yeasts, for molds

• Synonym(s): Selective media for yeasts and molds, Yeasts and molds medium
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• form: medium granules (dehydrated (DCM))
• Quality Level: 200
• packaging: pkg of 5 kg; pkg of 500 g
• manufacturer/tradename: GranuCult^®
• storage condition: protect from light (Tightly closed. Dry)
• technique(s): microbiological culture: suitable
• color: beige
• pH: 5.0 (30 °C, 33 g/L in H₂O, after autoclaving)
• solubility: 33 g/L
• bulk density: 650 kg/m³
• application(s): food and beverages; microbiology
• storage temp.: 15-25°C
• suitability: molds; yeasts

Description

General description

Wort broth (base) is used for the cultivation, isolation, and enumeration or enrichment of yeasts. The addition of certain indicator dyes to the Wort broth base allows differentiation between yeast and bacterial colonies. The accompanying bacterial flora is weakly suppressed by the pH value of 5.0 and largely by a pH of 3.5.

Application

Wort broth (base) is suitable for the cultivation, isolation, and enumeration or enrichment of fungi, especially yeasts.

Features and Benefits

• GranuCult^® offers superior granulated culture media
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): clear
Appearance (colour): yellowish-brown
pH-value (25 °C): 4.8 - 5.2
Growth (Candida albicans ATCC 10231 (WDCM 00054)): good/very good
Growth (Saccharomyces cerevisiae ATCC 9763 (WDCM 00058)): good/very good
Growth (Saccharomyces cerevisiae ATCC 9080): good/very good
Growth (Geotrichum candidum DSM 1240): good/very goog
Growth (Rhodotorula mucilaginosa DSM 70403): good/very good
Growth (Penicillium commune ATCC 10428): good/very good
Growth (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): good/very good
Growth (Trichophyton ajelloi ATCC 28454): good/very good
Incubation: 7 days; 28 °C; aerobic.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3