Skip to Content
Merck
All Photos(1)

Key Documents

Safety Information

1.02572

Sigma-Aldrich

Schiff′s reagent Intense

for the detection of aldehyde and mucosubstances in microscopy

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41116130
NACRES:
NA.41

Quality Level

400

form

liquid

IVD

for in vitro diagnostic use

pH

1.74 ( in H2O)

density

1.014 g/cm3 at 20 °C

storage temp.

20-25°C

General description

The Schiff′s reagent Intense - for the detection of aldehyde and mucosubstances in microscopy, is a ready-to-use solution used for human-medical cell diagnosis and serves the histological investigation of sample material of human origin, for example histological sections of e. g. the intestine or liver. This ready-to-use Schiff′s reagent Intense can be used to stain mucopolysaccharides in histological tissue specimens. The PAS (periodic acid schiff) reaction is one of the most frequently used chemical methods for the detection of aldehyde and mucosubstances in microscopy. In the PAS reaction, the histological specimen material is first treated with periodic acid, resulting in the oxidation of the 1,2-glycols into aldehyde groups. The addition of Schiff′s reagent (fuchsin-sulfuric acid) in the second step causes the aldehydes to react to form a brilliant red color. In the end result, the PAS reaction yields a specific color reaction with unsubstituted polysaccharides, neutral mucopolysaccharides, muco- and glycoproteins, and glyco- and phospholipids.
The PAS reaction can be further combined with the Alcian blue staining method to detect mucosubstances (glycosaminoglycans).
The 500 ml bottle is sufficient for 3000 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Application

For the detection of aldehydes, polysaccharides (glycogen), and mucosubstances (glycoproteins, glycolipids, mucins)

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 1

1 of 1

Periodic acid ReagentPlus®, ≥99.0%

Sigma-Aldrich

P7875

Periodic acid

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service