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1.00950

Millipore

Chromocult® Enterococci-Agar

Selective culture medium for the isolation and differentiation of Enterococci

Synonym(s):

Enterococcus Agar, Chromogenic Enterococci agar

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About This Item

UNSPSC Code:
41106213
NACRES:
NA.74

Quality Level

200

form

medium granules (dehydrated (DCM))

manufacturer/tradename

GranuCult®

technique(s)

microbiological culture: suitable

pH

6.8-7.2 (25 °C, 33 g/L in H2O)

solubility

33 g/L

application(s)

food and beverages
microbiology

storage temp.

15-25°C

suitability

Enterococcus spp.

Related Categories

General description

The presence of Enterococci, especially E. faecalis, E. faecium, E. durans and E. hirae, serves as an indicator for fecal contamination. The Enterococci-Agar medium is provided with a unique chromogenic substrate that is acted on by various Enterococci species to produce red colonies that can easily be identified. Non-Enterococci species produce blue/violet, turquoise or even colorless colonies which can easily be distinguished from the red. The medium is also provided with sodium azide and ox bile which inhibits the growth of any accompanying microbial flora.

Application

Chromocult® Enterococci-Agar can be used to isolate, differentiate and enumerate Enterococci present in various foodstuff, water or animal feed. It can also be used as for determination of fecal contamination in foodstuff, water or animal feed

Analysis Note

Appearance (clearness): clear
Appearance (color): yellowish
pH-value (25 °C): 6.8 - 7.2
Recovery on test medium (Enterococcus faecium ATCC 882): ≥ 50 %
Recovery on test medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 70 %
Recovery on test medium (Enterococcus durans ATCC 6056): ≥ 70 %
Recovery on test medium (Enterococcus hirae ATCC 8043 (WDCM 00089)): ≥ 70 %
Recovery on test medium (Bacillus cereus ATCC 11778 (WDCM 00001)): ≤ 0.01 %
Recovery on test medium (Escherichia coli ATCC 11775 (WDCM 00090)): ≤ 0.01 %
Recovery on test medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): ≤ 0.01 %
Colony color (Enterococcus faecium ATCC 882): red
Colony color (Enterococcus faecalis ATCC 19433 (WDCM 00009)): red
Colony color (Enterococcus durans ATCC 6056): red
Colony color (Enterococcus hirae ATCC 8043 (WDCM 00089)): red
Colony color (Aerococcus viridans ATCC 10400): pink-red to violet
Growth (Aerococcus viridans ATCC 10400): inhibited
Incubation: 24 hrs.; 35 °C; aerobic.

Legal Information

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Hazard Statements

H412

Precautionary Statements

P273 - P501

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Poisonous substance

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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