07-677-IS

Anti-acetyl-Histone H3 (Lys56) Antibody, Trial Size

from rabbit, purified by affinity chromatography

• Synonym(s): Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l, H3K56Ac, Histone H3 (acetyl K56), Histone 3, H3, Histone cluster 3, H3, Histone H3 K56ac, Histone H3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• technique(s): ChIP: suitable; dot blot: suitable; western blot: suitable
• NCBI accession no.: NP_003520
• UniProt accession no.: P68431
• shipped in: wet ice
• target post-translational modification: acetylation (Lys56)
• Gene Information: human ... HIST1H3F(8968)

Description

General description

Histone H3 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).

Specificity

This antibody recognizes Histone H3 acetylated at Lys56.

Immunogen

Epitope: Acetylated Lys56

KLH-conjugated linear peptide corresponding to human Histone H3 acetylated at Lys56.

Application

Anti-acetyl-Histone H3 (Lys56) Antibody, Trial Size/ Pack is a rabbit polyclonal antibody for detection of acetyl-Histone H3 (Lys56) also known as H3K56Ac, Histone H3 (acetyl K56) & has been validated in WB, ChIP.

Dot Blot (Specificity) Analysis: A representative lot detected acetyl-Histone H3 (Lys56) in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667).

Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated acetyl-Histone H3 (Lys56) from HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Quality

Evaluated by Western Blot in HeLa acid extract, sodium butyrate treated HeLa acid extract, colcemid treated HeLa acid extract, recombinant Histone H3, recombinant Histone H4, and recombinant Histone H2A.

Western Blot Analysis: A 1:405 dilution of this antibody detected acetyl-Histone H3 (Lys56) in sodium butyrate treated HeLa acid extract.

Target description

~17 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa acid extract, sodium butyrate treated HeLa acid extract, colcemid treated HeLa acid extract, recombinant Histone H3, recombinant Histone H4, and recombinant Histone H2A

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable