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07-2139

Sigma-Aldrich

Anti-Cysteine Sulfenic Acid Antibody

serum, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702

biological source

rabbit

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, rat, human

species reactivity (predicted by homology)

mammals

technique(s)

western blot: suitable

shipped in

wet ice

target post-translational modification

unmodified

General description

Protein sulfenic acid formation is a reversible post-translational modification that may be used to monitor protein oxidation on reactive cysteines within target proteins. This can be detected with protein sulfenic acid derivatised with dimedone.

Specificity

This antibody recognizes proteins containing cysteine sulfenic acid derivitized with dimedone.

Immunogen

Epitope: Other
KLH-conjugated corresponding to cysteine sulfenic acid.

Application

Anti-Cysteine Sulfenic Acid detects levels of Cysteine Sulfenic Acid proteins & has been published & validated for use in WB.
Research Category
Apoptosis & Cancer
Research Sub Category
General Post-translation Modification
Western Blot Analysis: A previous lot of this antibody detected cysteine sulfenic acid in NIH/3T3 and HEK293 cell lysates.

Quality

Evaluated by Western Blot in rat ventricular myocyte lysate.

Western Blot Analysis: 1:10,000 dilution of this antibody detected cysteine sulfenic acid on 10 µg of rat ventricular myocyte lysate.

Target description

Multiple kDa are observed that increases with oxidized stress (H2O2).

Physical form

Unpurified
Unpurified rabbit polyclonal with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Rat ventricular myocyte lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

07-2139:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hayato Irokawa et al.
The Biochemical journal, 478(7), 1453-1470 (2021-03-23)
Redox regulation of proteins via cysteine residue oxidation is involved in the control of various cellular signal pathways. Pyruvate kinase M2 (PKM2), a rate-limiting enzyme in glycolysis, is critical for the metabolic shift from glycolysis to the pentose phosphate pathway
Claire Maller et al.
Antioxidants & redox signaling, 14(1), 49-60 (2010-06-04)
Protein sulfenic acids (SOHs) are the principal oxidation products formed when redox active proteins interact with peroxide molecules. We have developed a new antibody reagent that detects protein SOHs derivatized with dimedone. Using this new antibody, we found that glyceraldehyde

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