Skip to Content
Merck
All Photos(1)

Key Documents

Safety Information

07-1492

Sigma-Aldrich

Anti-Calcineurin Aα Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

NFAT transcription complex cytosolic component, nuclear factor of activated T-cells, cytoplasmic,, calcineurin-dependent 1, nuclear factor of activated T-cells, cytosolic component 1

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, mouse, human

species reactivity (predicted by homology)

mammals (based on 100% sequence homology)

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PPP3CA(5530)
mouse ... Ppp3Ca(19055)
rat ... Ppp3Ca(24674)

General description

Calcineurin is a serine/threonine protein phosphatase involved in the mediation of signal transduction in a wide variety of cellular responses. Calcineurin has been linked to Pmk1 MAP kinase and PI pathways, as well as to members of the small GTPase Rab/Ypt family and Type II myosin. Furthermore, calcineurin participates in the transduction of extracellular signals to the nucleus by dephosphorylation of members of the NFAT family of transcription factors. These linkages demonstrate calcineurin′s multifunctional capabilities in the regulation of calcium signaling. The Aα form of calcineurin is a major catalytic subunit gene isoform of calcineurin in most tissues that allows differentiation of Aα from the Aβ and Aγ isoforms.

Specificity

This antibody recognizes the Aα form of calcineurin.

Immunogen

KLH-conjugagted synthetic peptide corresponding to Calcineurin Aα.

Application

Research Category
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
This Anti-Calcineurin Aα Antibody is validated for use in WB for the detection of Calcineurin Aα.

Quality

Evaluated by Western Blot in Mouse brain lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected Calcineurin on 10 µg of mouse brain lysate.

Target description

58 kDa

Linkage

Replaces: AB1696

Physical form

Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 40% gylerol. Contains no preservative.

Storage and Stability

Store at -20°C for up to 12 months in undiluted aliquots; at this temperature the solution will
remain unfrozen.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Control
Mouse brain lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

07-1492:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Calcineurin regulates nuclear factor I dephosphorylation and activity in malignant glioma cell lines.
Brun, M; Glubrecht, DD; Baksh, S; Godbout, R
The Journal of Biological Chemistry null
Pradoldej Sompol et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(25), 6132-6148 (2017-06-01)
Hyperexcitable neuronal networks are mechanistically linked to the pathologic and clinical features of Alzheimer's disease (AD). Astrocytes are a primary defense against hyperexcitability, but their functional phenotype during AD is poorly understood. Here, we found that activated astrocytes in the

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service