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Key Documents

Safety Information

07-1450-I

Sigma-Aldrich

Anti-Beta Pix, SH3 Domain Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Rho guanine nucleotide exchange factor 7, Beta-Pix, COOL-1, PAK-interacting exchange factor beta, p85

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat, human

technique(s)

western blot: suitable

NCBI accession no.

NP_001106983

UniProt accession no.

Q14155

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ARHGEF7(8874)

General description

Beta-Pix (ARHGEF7, βPix, COOL) is a Rho guanine nucleotide exchange factor (GEF). Beta-Pix is a p21-activated protein kinase (PAK)-interacting exchange factor (Pix, COOL, p85SPR) that has been identified as a putative guanine nucleotide exchange factor (GEF) for Rac/Cdc42. The variants of Beta-Pix isoforms exhibit different tissue distributions. Beta-Pix-a is expressed ubiquitously in all the tissues examined, and other isoforms are expressed mainly in the central nervous system. PAK, a Ser/Thr kinase, is an important mediator of Rho family activity interacts with Beta-Pix at the SH3 domain of Beta-Pix though its proline rich region. This interaction is thought to affect the GTP hydrolysis rate of Rac and Cdc42.

Immunogen

Epitope: SH3 domain
GST-tagged recombinant protein corresponding to the SH3 domain of human Beta-Pix, SH3 Domain.

Application

Detect Beta Pix using this Anti-Beta Pix antibody, SH3 Domain validated for use in western blotting.
Research Category
Signaling
Research Sub Category
Signaling Neuroscience
Western Blotting Analysis: 1 µg/mL from a representative lot detected Beta-Pix, SH3 Domain in 10 µg of PC-12 cell lysate.

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Beta-Pix, SH3 Domain in 10 µg of Jurkat cell lysate.

Target description

~80 kDa observed

Linkage

Replaces: 07-1450

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

07-1450-I:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hideaki Ito et al.
Oncogene, 39(9), 1931-1943 (2019-11-23)
Cell motility is a tightly regulated phenomenon that supports the accurate formation of organ structure during development and homeostasis, including wound healing and inflammation. Meanwhile, cancer cells exhibit dysregulated motility, which causes spreading and invasion. The Dbl family RhoGEF ARHGEF7/β-PIX
Kunrong Cheng et al.
American journal of physiology. Gastrointestinal and liver physiology, 320(4), G627-G643 (2021-02-11)
Rho guanine nucleotide exchange factors (RhoGEFs) regulate Rho GTPase activity and cytoskeletal and cell adhesion dynamics. βPix, a CDC42/RAC family RhoGEF encoded by ARHGEF7, is reported to modulate human colon cancer cell proliferation and postwounding restitution of rat intestinal epithelial
Gulcan Semra Sahin et al.
Science signaling, 14(683) (2021-05-20)
Developing hippocampal neurons undergo rapid synaptogenesis in response to neurotrophic signals to form and refine circuit connections. The adipokine leptin is a satiety factor with neurotrophic actions, which potentiates both glutamatergic and GABAergic synaptogenesis in the hippocampus during neonatal development.
Sara K Donnelly et al.
The Journal of cell biology, 216(12), 4331-4349 (2017-10-25)
The initial step of metastasis is the local invasion of tumor cells into the surrounding tissue. Invadopodia are actin-based protrusions that mediate the matrix degradation necessary for invasion and metastasis of tumor cells. We demonstrate that Rac3 GTPase is critical

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