06-418
Anti-NFκB p65 Antibody, CT
Upstate®, from rabbit
Synonym(s):
Nuclear factor NF-kappa-B p65 subunit, v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear
factor of kappa light polypeptide gene enhancer in B-cells 3 (p65)), v-rel reticuloendotheliosis viral oncogene homolog A (avian), v-rel r
About This Item
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat, human
manufacturer/tradename
Upstate®
technique(s)
electrophoretic mobility shift assay: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
wet ice
target post-translational modification
unmodified
Gene Information
human ... NFKB1(4790)
General description
Specificity
Immunogen
Application
4 µg of a previous lot immunoprecipitated NFκB p65 from 500 μg of a human A431 carcinoma cell lysate.
Epigenetics & Nuclear Function
Transcription Factors
Quality
Western Blot Analysis:
0.5-2 µg/mL of this lot detected NFkB p65 in RIPA lysates from A431 cells.
Target description
Linkage
Physical form
Storage and Stability
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Analysis Note
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Other Notes
Legal Information
Disclaimer
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recommended
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
06-418:
Certificates of Analysis (COA)
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