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Key Documents

Safety Information

06-413

Sigma-Aldrich

Anti-NFκB p52 Antibody

Upstate®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

using Protein A

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_019408

UniProt accession no.

Q00653

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NFKB1(4790)

General description

The NFκB transcription factor was originally identified as a protein complex consisting of a 65 kDa DNA binding subunit and an associated 50 kDa protein. The 65 kDa subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFκB. It is synthesized as a 100 kDa protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFκB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells.

Specificity

Recognizes NFκB p52 and its precursor.

Immunogen

KLH conjugated synthetic peptide (CYNPGLDGIIEYDDFK) contained within amino acids 4-19 of human NF κB, p52

Application

Detect NFκB p52 with Anti-NFκB p52 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in IP & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Quality

routinely evaluated by immunoblot on RIPA lysates from Raji cells

Target description

52 kDa, and precursor (100 kDa)

Physical form

Format: Purified
Protein A Purified immunoglobulin in in 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% NaN3 as a preservative.
Unpurified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Raji cell lysate, Jurkat whole cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

06-413:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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TWEAK induces NF-kappaB2 p100 processing and long lasting NF-kappaB activation.
Saitoh, T; Nakayama, M; Nakano, H; Yagita, H; Yamamoto, N; Yamaoka, S
The Journal of Biological Chemistry null
Down-regulation of Bcl-2 enhances estrogen apoptotic action in long-term estradiol-depleted ER(+) breast cancer cells.
R X-D Song, Z Zhang, G Mor, R J Santen
Apoptosis null
Epstein-Barr virus latent infection membrane protein 1 TRAF-binding site induces NIK/IKK alpha-dependent noncanonical NF-kappaB activation.
Luftig, M; Yasui, T; Soni, V; Kang, MS; Jacobson, N; Cahir-McFarland, E; Seed, B; Kieff, E
Proceedings of the National Academy of Sciences of the USA null
Beyond the enhanceosome: cluster of novel ?B sites downstream of the human IFN-? gene is essential for lipopolysaccharide-induced gene activation.
Goh, FG; Thomson, SJ; Krausgruber, T; Lanfrancotti, A; Copley, RR; Udalova, IA
Blood null
Aberrant NF-kappaB2/p52 expression in Hodgkin/Reed-Sternberg cells and CD30-transformed rat fibroblasts.
Nonaka, M; Horie, R; Itoh, K; Watanabe, T; Yamamoto, N; Yamaoka, S
Oncogene null
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