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Key Documents

Safety Information

06-238

Sigma-Aldrich

Anti-Gβ Antibody

serum, Upstate®

Synonym(s):

Anti-HG2C1, Anti-NEDHYDF, Anti-SSS4

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

serum

clone

polyclonal

species reactivity

mouse, human

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_002074

UniProt accession no.

P62879

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... GNB2(2783)

Specificity

Gβ subunits; recognizes both isoforms

Immunogen

Internal sequence of G-protein β subunit (KTREGNVRVSREL) conjugated to octavalent polylysine base

Application

Detect Gβ using this Anti-Gβ Antibody validated for use in IP & WB.

Quality

Immunoblot Analysis: A 1:500-1:1000 dilution of this antibody detected the Gβ subunits in 20 μg of a cell lysate prepared from rat brain microsomal preparation (Catalog # 12-144).

Immunoprecipitation/Immunoblot: 10 μl of this antibody immunoprecipitated the Gβ subunit from 500 μg-1mg of a lysate of rat brain microsomal preparation (Catalog # 12-144).

Target description

35/36kDa

Physical form

PBS, pH 7.2

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

06-238:

Certificates of Analysis (COA)

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Importance of the G protein gamma subunit in activating G protein-coupled inward rectifier K(+) channels.
T Kawano, L Chen, S Y Watanabe, J Yamauchi, Y Kaziro, Y Nakajima, S Nakajima, H Itoh
Febs Letters null
M C Galas et al.
The Journal of biological chemistry, 272(5), 2788-2793 (1997-01-31)
The ADP-ribosylation factor (ARF) GTP-binding proteins are believed to function as regulators of vesicular budding and fusion along the secretory pathway. To investigate the role of ARF in regulated exocytosis, we have examined its intracellular distribution in cultured chromaffin cells
H I Krieger-Brauer et al.
The Journal of biological chemistry, 272(15), 10135-10143 (1997-04-11)
Human fat cells possess a multireceptor-linked H2O2-generating system that is activated by insulin. Previous studies revealed that manganese was the sole cofactor required for a hormonal regulation of NADPH-dependent H2O2 generation in vitro. In this report it is shown that
J Yamauchi et al.
The Journal of biological chemistry, 272(44), 27771-27777 (1997-11-05)
Various extracellular stimuli activate three classes of mitogen-activated protein kinases (MAPKs): extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38 MAPK. In mammalian cells, p38 MAPK is activated by endotoxins, inflammatory cytokines, and environmental stresses. We show here that p38 MAPK
J Yamauchi et al.
The Journal of biological chemistry, 274(4), 1957-1965 (1999-01-16)
Heterotrimeric G protein beta gamma subunit (Gbeta gamma) mediates signals to two types of stress-activated protein kinases, c-Jun NH2-terminal kinase (JNK) and p38 mitogen-activated protein kinase, in mammalian cells. To investigate the signaling mechanism whereby Gbeta gamma regulates the activity
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