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Safety Information

06-1306

Sigma-Aldrich

Anti-Mi-2b (CHD4) Antibody

serum, from rabbit

Synonym(s):

ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, chromodomain helicase DNA binding protein 4

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
clone:
polyclonal
application:
WB
species reactivity:
human
technique(s):
western blot: suitable
citations:
2

biological source

rabbit

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology)

technique(s)

western blot: suitable

NCBI accession no.

NP_666091.1

UniProt accession no.

Q6PDQ2

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CHD4(1108)

General description

CHD-4 is also often referred to as Mi2-beta. This protein is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. CHD4 has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. This interaction which depends on sumoylation of KLF1 leads to its transcriptional repression.

Specificity

Rat (96%).
This antibody recognizes CHD-4 at the N-terminus.

Immunogen

Epitope: N-terminus
GST-tagged recombinant protein corresponding to mouse CHD-4 at and around the N-terminus.

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Use Anti-Mi-2b (CHD4) Antibody (rabbit polyclonal antibody) validated in WB to detect Mi-2b (CHD4) also known as ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, chromodomain helicase DNA binding protein 4.

Quality

Evaluated by Western Blot in HeLa nuclear extract cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected CHD-4 on 10 µg of HeLa nuclear extract cell lysate.

Target description

217 kDa

Physical form

Unpurified
Unpurified rabbit polyclonal in serum with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HeLa nuclear extract cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

06-1306:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Xiaofei Yu et al.
Haematologica, 104(12), 2361-2371 (2019-04-21)
As high fetal hemoglobin levels ameliorate the underlying pathophysiological defects in sickle cell anemia and beta (β)-thalassemia, understanding the mechanisms that enforce silencing of fetal hemoglobin postnatally offers the promise of effective molecular therapy. Depletion of the Nucleosome Remodeling and

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