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Key Documents

Safety Information

05-916

Sigma-Aldrich

Anti-P-Cadherin Antibody, clone 6A9

ascites fluid, clone 6A9, Upstate®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

6A9, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NM_001793

UniProt accession no.

P22223

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... CDH3(1001)

Specificity

Recognizes P-Cadherin

Immunogen

P-Cadherin extracellular domain purified from A431 cell membranes.

Application

This Anti-P-Cadherin Antibody, clone 6A9 is validated for use in IP, WB, IC for the detection of P-Cadherin.

Quality

Routinely evaluated by immunoblot on A431 cell lysates.

Target description

Mr 120kDa

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

05-916:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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K A Knudsen et al.
Human pathology, 31(8), 961-965 (2000-09-15)
Breast cancers often show reduced expression of the transmembrane cell-cell adhesion protein, E-cadherin. In addition, approximately half of breast carcinomas express P-cadherin, which correlates with poor survival. A large fragment of the E-cadherin extracellular domain can be detected in serum
M D Hines et al.
Journal of cell science, 112 ( Pt 24), 4569-4579 (1999-11-27)
Cadherin function is required for normal keratinocyte intercellular adhesion and stratification. In the present study, we have investigated whether cadherin-cadherin interactions may also modulate keratinocyte differentiation, as evidenced by alterations in the levels of several differentiation markers. Confluent keratinocyte cultures
Cadherin function is required for human keratinocytes to assemble desmosomes and stratify in response to calcium
Lewis, J. E., et al
The Journal of Investigative Dermatology, 102, 870-877 (1994)
M T Nieman et al.
Journal of cell science, 112 ( Pt 10), 1621-1632 (1999-04-23)
The cadherin/catenin complex mediates Ca2+-dependent cell-cell interactions that are essential for normal developmental processes. It has been proposed that sorting of cells during embryonic development is due, at least in part, to expression of different cadherin family members or to
Identification of human embryonic stem cell surface markers by combined membrane-polysome translation state array analysis and immunotranscriptional profiling.
Kolle G, Ho M, Zhou Q, Chy HS, Krishnan K, Cloonan N, Bertoncello I, Laslett AL, Grimmond SM
Stem Cells null
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