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Safety Information

05-666

Sigma-Aldrich

Anti-BRCA2 Antibody, clone 5.23

clone 5.23, Upstate®, from mouse

Synonym(s):

Anti BRCC2, Anti-BROVCA2, Anti-FACD, Anti-FAD, Anti-FAD1, Anti-FANCD, Anti-FANCD1, Anti-GLM3, Anti-PNCA2, Anti-XRCC11

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

5.23, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_000059

UniProt accession no.

P51587

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... BRCA2(675)

Specificity

BRCA2

Immunogen

GST fusion protein to residues 2959-3418 of human BRCA2 protein

Application

Detect BRCA2 with Anti-BRCA2 Antibody, clone 5.23 (Mouse Monoclonal Antibody), that has been shown to work in WB, ICC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

Quality

routinely evaluated by immunoblot on in HeLa nuclear extract

Target description

390kDa

Physical form

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, and 0.05% sodium azide.
Format: Purified
Protein G Chromatography

Storage and Stability

Store at 2-8°C. Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at 2-8°C.

Analysis Note

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

Other Notes

Due to license agreement restrictions, this product cannot be purchased for resale.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

05-666:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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BLM SUMOylation regulates ssDNA accumulation at stalled replication forks.
Ouyang, KJ; Yagle, MK; Matunis, MJ; Ellis, NA
Frontiers in Genetics null
Loredana Moro et al.
The Journal of biological chemistry, 280(23), 22482-22491 (2005-04-05)
BRCA2 is a tumor suppressor gene that when mutated confers an increased susceptibility to developing breast and prostate carcinoma. Besides its role in mediating DNA repair, new evidence suggests that BRCA2 may also play a role in suppressing cancer cell
Loredana Moro et al.
The Journal of biological chemistry, 281(31), 22100-22107 (2006-06-07)
Aberrant interaction of carcinoma cells with basement membranes (BM) is a fundamental pathophysiological process that initiates a series of events resulting in cancer cell invasion and metastasis. In this report, we describe the results of our investigations pertaining to the
Miyoung Lee et al.
Nucleic acids research, 49(10), 5588-5604 (2021-05-13)
Cancer-causing missense mutations in the 3418 amino acid BRCA2 breast and ovarian cancer suppressor protein frequently affect a short (∼340 residue) segment in its carboxyl-terminal domain (DBD). Here, we identify a shared molecular mechanism underlying their pathogenicity. Pathogenic BRCA2 missense
L Y Marmorstein et al.
Cell, 104(2), 247-257 (2001-02-24)
Germline mutations of the human BRCA2 gene confer susceptibility to breast cancer. Although the function of the BRCA2 protein remains to be determined, murine cells homozygous for BRCA2 inactivation display chromosomal aberrations. We have isolated a 2 MDa BRCA2-containing complex
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