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Key Documents

Safety Information

05-636-AF488

Sigma-Aldrich

Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor™ 488 Conjugate Antibody

clone JBW301, from mouse, ALEXA FLUOR™ 488

Synonym(s):

Histone H2AX, H2a/x, Histone H2A.X

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR™ 488

antibody form

purified antibody

antibody product type

primary antibodies

clone

JBW301, monoclonal

species reactivity

human

species reactivity (predicted by homology)

vertebrates (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable

isotype

IgG1

NCBI accession no.

NP_002096

UniProt accession no.

P16104

shipped in

wet ice

target post-translational modification

phosphorylation (pSer139)

Gene Information

human ... H2AX(3014)

General description

Histone H2A.X is a variant of histone H2A, and is similarly associated with genomic DNA. However, histone is structurally different from other members of the H2A family in possessing a C-terminal tail that contains the Ser139 residue that is phosphorylated in response to breaks in double-stranded DNA. The phosphorylation of H2A.X is a rapid process that is mediated by ATM/ATR proteins.

Application

This Anti-phospho Histone H2A.X (Ser139) antibody, clone JBW301, Alexa Fluor™ 488 Conjugate is validated for use in ICC for the detection of phospho Histone H2A.X (Ser139).

Quality

Evaluated by Immunocytochemistry in untreated (negative control) and Staurosporin treated HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-Histone H2A.X (Ser139) in Staurosporin treated HeLa cells, and not in untreated HeLa cells.

Target description

17 kDa calculated

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

05-636-AF488:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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