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Key Documents

Safety Information

05-561

Sigma-Aldrich

Anti-LAT Antibody, clone 2E9

clone 2E9, Upstate®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

2E9, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_014387

UniProt accession no.

O43561

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... LAT(27040)

Specificity

Recognizes both forms of LAT.

Immunogen

GST fusion protein corresponding to residues 31-233 of human LAT

Application

Detect LAT using this Anti-LAT Antibody, clone 2E9 validated for use in IP & WB.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Quality

routinely evaluated by immunoblot on RIPA lysates from Jurkat cells

Target description

36/38 kDa

Physical form

Format: Purified
Protein A Purified immunoglobulin in Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl and 0.05% Sodium Azide.
Protein G Purified

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

05-561:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nupura Hirve et al.
PloS one, 7(9), e45158-e45158 (2012-10-03)
Binding of the membrane phospholipid phosphatidylinositol 3,4,5-trisphosphate (PIP(3)) to the Pleckstrin Homology (PH) domain of the Tec family protein tyrosine kinase, Inducible T cell Kinase (ITK), is critical for the recruitment of the kinase to the plasma membrane and its
W Zhang et al.
Cell, 92(1), 83-92 (1998-03-07)
Despite extensive study, several of the major components involved in T cell receptor-mediated signaling remain unidentified. Here we report the cloning of the cDNA for a highly tyrosine-phosphorylated 36-38 kDa protein, previously characterized by its association with Grb2, phospholipase C-gamma1

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