05-412
Anti-GSK3 Antibody, clone 4G-1E
clone 4G-1E, Upstate®, from mouse
Synonym(s):
GSK-3 alpha, glycogen synthase kinase 3 alpha
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
4G-1E, monoclonal
species reactivity
mouse, Dictyostelium sp., Schizosaccharomyces pombe, rat, vertebrates, human
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1
suitability
not suitable for immunoprecipitation
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... GSK3A(2931)
mouse ... Gsk3A(606496)
rat ... Gsk3A(50686)
General description
Glycogen synthase kinase-3 (GSK-3), a multifunctional serine/threonine kinase, is a key regulator of numerous signaling pathways. Two isoforms of GSK-3 are reported in mammals: a 51 kDa GSK-3a and a 47 kDa GSK-3b. These two isoforms exhibit about 98% homology in their kinase domains, but share only about 36% identity in the last 76 C-terminal amino acid residues. GSK-3a contains a glycine-rich extension at its N-terminus. A minor (~15% of total) splice variant of GSK-3b, GSK-3b2, has also been identified, which contains a 13-residue insert within the kinase domain. It exhibits reduced kinase activity towards tau protein compared with ‘unspliced’ GSK-3b. GSK-3b2 is localized primarily to neuronal cell bodies, unlike unspliced GSK-3b that is also found in neuronal processes. GSK-3b plays a key inhibitory role in the Wnt signaling pathway. Wnt genes encode a large family of secreted, cysteine-rich proteins that are important in development and in maintenance of adult tissues. Abnormalities in Wnt signaling are reported to promote both human dgenerative diseases and cancer.
Specificity
Because of the extremely high conservation of this immunogen among the known enzymes of many different species, this antibody is predicted to cross-react with nearly all species homologues.
GSK3, α and β isoforms
Immunogen
18 residue synthetic peptide (C-KQLLHGEP NVSYICSRY) corresponding to amino acids 203-219 derived from a region of the catalytic domain (between subdomain VII and VIII) of the Drosophila GSK3/shaggy enzyme, coupled to ovalbumin. Clone 4G-1E.
Application
Anti-GSK3 Antibody, clone 4G-1E detects level of GSK3 & has been published & validated for use in IC & WB.
Immunoprecipitation:
Not recommended.
Immunocytochemistry:
10 µg/mL of a previous lot showed positive immunostaining for GSK3 in L6 cells fixed with acetone.
Not recommended.
Immunocytochemistry:
10 µg/mL of a previous lot showed positive immunostaining for GSK3 in L6 cells fixed with acetone.
Research Category
Signaling
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
PI3K, Akt, & mTOR Signaling
Quality
Routinely evaluated by immunoblot on human Jurkat cells, human A431, rat L6, rat PC12 or mouse 3T3 cells.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected GSK3 in RIPA lysates from human Jurkat cells; previously GSK3 was detected in RIPA lysates from human A431, rat L6 and PC12, and mouse 3T3 cells.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected GSK3 in RIPA lysates from human Jurkat cells; previously GSK3 was detected in RIPA lysates from human A431, rat L6 and PC12, and mouse 3T3 cells.
Target description
51 kDa (α) or 46 kDa (β)
Physical form
Format: Purified
Protein G Purified
Protein G purified mouse IgG1 in storage buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
05-412:
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Assays for glycogen synthase kinase-3 (GSK-3).
D Cross
Methods in molecular biology (Clifton, N.J.), 124, 147-159 (2000-12-02)
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