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Safety Information

05-382

Sigma-Aldrich

Anti-Na+/K+ ATPase β-1 Antibody, clone C464.8

clone C464.8, Upstate®, from mouse

Synonym(s):

Anti-AHC2, Anti-ATP1A1, Anti-CAPOS, Anti-DEE99, Anti-DYT12, Anti-RDP

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

C464.8, monoclonal

species reactivity

rabbit, bovine, canine, human, rat

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NM_001677

UniProt accession no.

P13637

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ATP1A3(478)

General description

Clone C464.8 is also referred to as clone 8A

Specificity

Recognizes the β-1 subunit isoform of Na+/K+.

Immunogen

Purified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla.

Application

Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
This Anti-Na+/K+ ATPase β-1 Antibody, clone C464.8 is validated for use in IH, WB for the detection of Na+/K+ ATPase β-1.

Quality

routinely evaluated in immunoblot on rat brain, rat heart or rat kidney microsomal preparations

Target description

broad band at an apparent molecular weight of 48-55 kDa due to glycosylation

Physical form

Format: Purified
Protein G Purified
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in PBS with 0.05% sodium azide before the addition of glycerol to 30%

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Expressed in most tissue, including brain, heart and kidney

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

05-382:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Motor neuron degeneration in amyotrophic lateral sclerosis mutant superoxide dismutase-1 transgenic mice: mechanisms of mitochondriopathy and cell death.
Lee J Martin,Zhiping Liu,Kevin Chen,Ann C Price,Yan Pan,Jason A Swaby,W Christopher Golden
The Journal of Comparative Neurology null
Man Liang et al.
The Journal of biological chemistry, 282(14), 10585-10593 (2007-02-14)
Recent studies have ascribed many non-pumping functions to the Na/K-ATPase. Here, we present experimental evidence demonstrating that over half of the plasma membrane Na/K-ATPase in LLC-PK1 cells is performing cellular functions other than ion pumping. This "non-pumping" pool of Na/K-ATPase
Regional distribution of Na,K-ATPase activity in porcine lens epithelium
Tamiya, S., et al
Investigative Ophthalmology & Visual Science, 44, 4395-4399 (2003)
Thyroid hormone stimulates Na-K-ATPase activity and its plasma membrane insertion in rat alveolar epithelial cells.
Jianxun Lei, Sogol Nowbar, Cary N Mariash, David H Ingbar
American Journal of Physiology. Lung Cellular and Molecular Physiology null
An ion-transporting ATPase encodes multiple apical localization signals.
Gottardi, C J and Caplan, M J
The Journal of cell biology, 121, 283-293 (1993)
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