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Safety Information

05-366

Sigma-Aldrich

Anti-PLCγ-1 Antibody, clone B-6-4

clone B-6-4, 1 mg/mL, Upstate®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

B-6-4, monoclonal

species reactivity

human, rabbit, rat, bovine, mouse

manufacturer/tradename

Upstate®

concentration

1 mg/mL

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_002660

UniProt accession no.

P19174

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PLCG1(5335)

Specificity

PLCγ-1; may also react with some non-PLC γ-1 SH-3 containing proteins

Immunogen

Purified soluble phospholipase C (PLC) g-1 from bovine brain

Application

Anti-PLCγ-1 Antibody, clone B-6-4 is an antibody against PLCγ-1 for use in IP & WB.
Research Category
Signaling
Research Sub Category
Lipid Signaling

Quality

Routinely evaluated in immunoblot using acid precipitated protein from bovine brain, RIPA lysates from human A431 cells, and previously using mouse 3T3/A31 fibroblasts and rat L6 myoblasts lysates.

Target description

~135 kDa

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1 antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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GHS07

Signal Word

Warning

Hazard Statements

H302 + H312 + H332,H412

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Poisonous substance

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

05-366:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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L Rui et al.
The Journal of biological chemistry, 274(15), 10590-10594 (1999-04-03)
Nerve growth factor (NGF) is essential for the development and survival of sympathetic and sensory neurons. NGF binds to TrkA, activates the intrinsic kinase activity of TrkA, and promotes the differentiation of pheochromocytoma (PC12) cells into sympathetic-like neurons. Several signaling
T Fukazawa et al.
The Journal of biological chemistry, 270(34), 20177-20182 (1995-08-25)
Tyrosine phosphorylation of cellular proteins is an early and an essential step in T cell receptor-mediated lymphocyte activation. Tyrosine phosphorylation of transmembrane receptor chains (such as zeta and CD3 chains) and membrane-associated proteins provides docking sites for SH2 domains of
Phospholipase C and src tyrosine kinases mediate neurotensin-stimulated Cl- secretion in rabbit proximal colon.
Roli Prasad, Jayashree Venkatasubramanian, Milen Amde, Mrinalini Chatta Rao
Digestive Diseases and Sciences null
Tyrosine phosphorylation and recruitment of ZAP-70 to the CD3-TCR complex are defective after CD2 stimulation
Hubert, P., et al
Journal of immunology (Baltimore, Md. : 1950), 157, 4322-4332 (1996)
CD3 stimulation causes phosphorylation of phospholipase C-gamma 1 on serine and tyrosine residues in a human T-cell line.
Park, D J, et al.
Proceedings of the National Academy of Sciences of the USA, 88, 5453-5456 (1991)
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