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Safety Information

05-347

Sigma-Aldrich

Anti-PCNA Antibody, clone PC10

clone PC10, Upstate®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

culture supernatant

antibody product type

primary antibodies

clone

PC10, monoclonal

species reactivity

vertebrates

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NM_002592

UniProt accession no.

P12004

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PCNA(5111)

General description

Expression of proliferating cell nuclear antigen (PCNA), cyclin or polymerase delta auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. PCNA/cyclin may act as an auxiliary protein of DNA polymerase-delta to play a fundamental role in the initiation of cell proliferation. Anti-PCNA reacts with PCNA at the molecular weight of 36 kDa on western blot.

Specificity

PCNA

Immunogen

Recombinant rat PCNA

Application

Detect PCNA with Anti-PCNA Antibody, clone PC10 (Mouse Monoclonal Antibody), that has been shown to work in WB, ICC & IHC.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

Quality

routinely evaluated by immunoblot on RIPA lysate from human A431 cells or mouse 3T3/A31 cell lysate

Target description

36kDa

Physical form

Concentrated culture supernatant
Format: Purified
Protein A purified mouse immunoglobulin in PBS containing 1 mg/mL BSA and 10mM Sodium Azide.

Storage and Stability

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

05-347:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Proliferating cell nuclear antigen is required for DNA excision repair.
Shivji, K K, et al.
Cell, 69, 367-374 (1992)
Adenosine reverses a preestablished CCl4-induced micronodular cirrhosis through enhancing collagenolytic activity and stimulating hepatocyte cell proliferation in rats
Hernandez-Munoz, R., et al
Hepatology, 34, 677-687 (2001)
Mechanism of elongation of primed DNA by DNA polymerase delta, proliferating cell nuclear antigen, and activator 1.
Lee, S H and Hurwitz, J
Proceedings of the National Academy of Sciences of the USA, 87, 5672-5676 (1990)
Xiangyuan Wang et al.
Fetal diagnosis and therapy, 29(4), 315-320 (2011-01-14)
We investigated the patterns of expression of HOXB5, cyclin D1 and proliferating cell nuclear antigen (PCNA) proteins in human congenital cystic adenomatoid malformation (CCAM) to establish the molecular basis of its etiology. Immunohistochemistry was performed on frozen archival specimens of
Yoshiyuki Shibata et al.
The Journal of biological chemistry, 277(1), 746-754 (2001-11-01)
Flap endonuclease 1 (FEN-1) is a 5'-3' flap exo-/endonuclease that plays an important role in Okazaki fragment maturation, nonhomologous end joining of double-stranded DNA breaks, and long patch base excision repair. Here, we demonstrate that the wild type FEN-1 binds
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