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Key Documents

05-1109

Sigma-Aldrich

Anti-Pro-Insulin C-Peptide Antibody, clone C-PEP-01

clone C-PEP-01, from mouse

Synonym(s):

CD220 antigen, insulin receptor

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

C-PEP-01, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin)

isotype

IgG1

UniProt accession no.

P01308

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... INS(3630)

General description

Pro-Insulin consists of the three parts: C-peptide and two long strands of amino acids (alpha and beta chains; later become linked together to form the Insulin molecule). From every molecule of Pro-Insulin, one molecule of Insulin plus one molecule of C-peptide are produced. C-peptide is released into the blood stream in equal amounts to Insulin.

Specificity

No cross-reactivity with Insulin or other peptide hormones or proteins was observed.
The antibody C-PEP-01 reacts specifically with C-peptide, a part of the Pro-insulin molecule.

Immunogen

Synthetic C-peptide of human pro-insulin conjugated to bovine serum albumin.

Application

Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling
This Anti-Pro-Insulin C-Peptide Antibody, clone C-PEP-01 is validated for use in IH(P) for the detection of Pro-Insulin C-Peptide.

Quality

Evaluated by Immunohistochemistry in normal human pancreas.
Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected insulin in human normal pancreas tissue.

Target description

Approx. 12 kDa

Linkage

Replaces: CBL94

Physical form

Format: Purified
Mouse monoclonal IgG1 in phosphate buffered saline, pH7.4, and 15 mM sodium azide.
Sequential precipitation with caprylic acid and ammonium sulfate.

Storage and Stability

Store at 2-8°C and use within 1 year from date of receipt.

Analysis Note

Control
Human normal pancreas tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sapna Puri et al.
Nature communications, 9(1), 485-485 (2018-02-06)
Pancreatic β cells are highly specialized to regulate systemic glucose levels by secreting insulin. In adults, increase in β-cell mass is limited due to brakes on cell replication. In contrast, proliferation is robust in neonatal β cells that are functionally
Human oocytes reprogram adult somatic nuclei of a type 1 diabetic to diploid pluripotent stem cells.
Yamada, Mitsutoshi, et al.
Nature (2014)
Haiqing Hua et al.
The Journal of clinical investigation, 123(7), 3146-3153 (2013-06-20)
Diabetes is a disorder characterized by loss of β cell mass and/or β cell function, leading to deficiency of insulin relative to metabolic need. To determine whether stem cell-derived β cells recapitulate molecular-physiological phenotypes of a diabetic subject, we generated
Bipasha Bose et al.
Methods in molecular biology (Clifton, N.J.), 1341, 257-284 (2015-03-19)
Since the advent of pluripotent stem cells, (embryonic and induced pluripotent stem cells), applications of such pluripotent stem cells are of prime importance. Indeed, scientists are involved in studying the basic biology of pluripotent stem cells, but equal impetus is

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