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Safety Information

03-903

Sigma-Aldrich

GFP, FLAG-tagged SNRNP70 mRNA

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.52

packaging

1 ea of 50 μg

Quality Level

100

manufacturer/tradename

Millipore

growth mode

N/A

technique(s)

RIP: suitable
cell culture | mammalian: suitable
flow cytometry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

shipped in

dry ice

storage temp.

-65 to -96°C

Application

Both mRNAs and non-coding RNAs interact with RNA-binding proteins and regulate critical cellular processes and their dysregulation contributes to multiple diseases. Thus, analyzing RNA-protein interactions are critical for understanding the mechanism of diseases and designing clinical strategies.
RNA-binding protein immunoprecipitation (RIP) has been used to interrogate RNA binding proteins (RBP) associated with specific RNA molecules. However, the experiments are not feasible because of the unavailability of antibodies for the particular RBP or low expression of the RBP. To overcome these issues epitope tags have been used for RIP experiments.

Features and Benefits

The GFP, FLAG-tagged SNRNP70 mRNA was developed to enable to perform control RIP experiments with RIPAb+ FLAG (Cat. No. 03-901) or RIPAb+ GFP (Cat. No. 03-902) RIP validated antibody and primer kits, and Magna RIP kit (Cat. # 17-700).

Storage and Stability

Store at -80ºC

Other Notes

The high amount of GFP-FLAG-tagged SNRNP70 protein is expressed immediately after transfection to cells and the well-known specific interaction of SNRNP70 and U1snRNA can be used for control RIP experiments with anti-GFP or anti-FLAG antibodies.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

03-903:

Certificates of Analysis (COA)

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