03-0206-00

SMC^® Human SNAP-25 High Sensitivity Immunoassay Kit

1 kit sufficient for 96 wells, input: serum, plasma

• Synonym(s): SNAP-25 Single Molecule Counting kit, SNAP-25 immunoassay kit, SNAP-25 ultra-sensitive immunoassay, Synaptosomal-associated protein 25 immunoassay kit
• UNSPSC Code: 12161503
• NACRES: NA.77

Properties

• Quality Level: 200
• usage: kit sufficient for 96 wells
• species reactivity: human
• manufacturer/tradename: SMC^®
• assay range: sensitivity: 0.26 pg/mL; (lower limit of quantification)
• technique(s): single molecule counting: suitable
• input: serum; plasma
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

The SMC^® Human SNAP-25 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences:

1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMCxPRO^® immunoassay systems detects analytes using Single Molecule Counting (SMC^®) technology.
Human Synaptosomal-Associated Protein, 25kDa (SNAP-25) is a protein receptor expressed mainly in developing neural tissue, and is a component of the trans-SNARE complex, which facilitates the exocytosis and release of synaptic vesicles containing neurotransmitters into the synaptic cleft of neurons. SNAP-25 has been recently studied as a potential biomarker for synaptic dysfunction in general, with relevance in psychiatric diseases such as attention deficiency hyperactivity disorder (ADHD), schizophrenia, and bipolar disorders. Additionally, the decrease of this presynaptic protein is being evaluated as a predictor of neurodegenerative conditions involving cognitive loss, such as Alzheimer’s disease. The new SMC^® Human SNAP-25 High Sensitivity Immunoassay Kit enables accurate measurements of this protein in cerebrospinal fluid (CSF), serum, and plasma samples even at low abundance, and empowers work aimed to probe disease mechanisms and develop potential therapies for both psychiatric and neurodegenerative disorders.

Research Category:
Neuroscience
neurological


Research Sub-Category:
Alzheimer′s Disease
Aging
Nervous System
Neurodegenerative Diseases
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Neuroregenerative Medicine
Neuroscience

Specificity

SMCxPRO^® instrument response above background was detected using this assay when measuring samples from the following species:
Non-Human Primate (n=4)
Canine (n=4)
Feline (n=4)
Rat (n=4)
Mouse (n=4)
Note: This assay was tested for cross-reactivity with SNAP-23, STX1A, and NFL. No cross-reactivity was detected with any of these analytes.

Application

The sensitivity of the Single Molecule Counting SMC^® technology gives the SMC^® Human SNAP-25 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of Human SNAP-25 in human serum, plasma, and CSF.

Features and Benefits

• Improved options for sample prep and incubation
• Lyophilization protein standard for uniform shipping and storage condition
• Increased reagent volumes to accommodate automation
• Rigorously developed to achieve accurate and reproducible performance on the state-of-the-art SMCxPRO^® ultrasensitive immunoassay system.
  

Components

• Assay Buffer
• SNAP-25 Coated Beads
• Standard Diluent
• SNAP-25Detection Antibody
• SNAP-25 Standard
• 10X Wash Buffer
• Buffer D
• Elution Buffer B
  

Specifications

Minimum of 4 serum samples spiked with average recovery of 82% with a range of 71-95%
Minimum of 4 plasma samples spiked with average recovery of 91% with a range of 77-99%
Minimum of 5 CSF samples spiked with average recovery of 89% with a range of 83-103%

Legal Information

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS06,GHS08
• Signal Word: Danger
• Hazard Statements: H302 + H332,H311,H319,H360FD,H412
• Precautionary Statements: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects