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810603P

Avanti

16:0-10 Doxyl PC

Avanti Research™ - A Croda Brand 810603P, powder

Synonym(s):

1-palmitoyl-2-stearoyl-(10-doxyl)-sn-glycero-3-phosphocholine

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About This Item

Empirical Formula (Hill Notation):
C46H90N2O10P
CAS Number:
188004-25-3
Molecular Weight:
862.19
UNSPSC Code:
41141825
NACRES:
NA.25

Assay

>99% (TLC)

form

powder

packaging

pkg of 1 × 1 mg (810603P-1mg)

manufacturer/tradename

Avanti Research™ - A Croda Brand 810603P

lipid type

ESR probes
phospholipids

shipped in

dry ice

storage temp.

−20°C

General description

Avanti′s nitroxide spin product listing is a group of compounds designed to act as membrane probes. A variety of positions down the hydrophobic chain are labeled with the nitroxide functional groups to allow probing the membrane at various depths. These compounds have been synthesized from 1-palmitoyl-2-hydroxy-sn-glycerol-3-phosphocholine with the product being purified by column chromatography. Various n-doxyl phosphocholines have been recently used as biophysical tools to elucidate membrane trafficking with phosphatidylinositol transfer proteins and as fluorescent quenchers in lipid bilayer structural studies.
Phosphocholine is considered as a precursor molecule. It is formed during the breakdown of phosphatidylcholine metabolism. 10-Doxyl is a spin label probe.

Application

16:0-10 Doxyl PC may be used:
  • in vesicles for quenching PT-(1–46)F4W fluorescence
  • as vesicle component for tryptophan based fluorescence emission studies
  • to probe the oxygen and Ni[II] ethylenediaminediacetic acid (NiEdda) accessibilities inside liposomes

Biochem/physiol Actions

Phosphatidylcholine (PC) functions as a surfactant within the mucus to form a hydrophobic surface to inhibit bacterial penetrance.

Packaging

5 mL Clear Glass Sealed Ampule (810603P-1mg)

Preparation Note

Product use: To prevent aggregation, prepare water-based solutions of 2 mM stock solutions of n-DOXYL PCs and store in plastic. Dilute stock solutions to 0.03- 0.1 mM solutions for EPR studies. For liposome preparations in fluorescent quenching measurements, dissolve the doxyl lipid in 150 μl absolute ethanol for a concentration of 40.3 mM , Additional supplemental information.

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Storage Class Code

11 - Combustible Solids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

810603P-1MG:
810603P-BULK:
810603P-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Integrative Medicine - E-Book (2017)
Alexander Kyrychenko et al.
The journal of physical chemistry. B, 117(19), 5875-5885 (2013-04-26)
Spin-labeled lipids are commonly used as fluorescence quenchers in studies of membrane penetration of dye-labeled proteins and peptides using depth-dependent quenching. Accurate calculations of depth of the fluorophore rely on the use of several spin labels placed in the membrane
Delivery of nitroxide spin label to cultured cells by liposomes
Chan HC
Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine, 8, 160-170 (1988)
Phosphatidylserine dynamics in cellular membranes.
Kay, J.G
Molecular Biology of the Cell, 23, 2198-2212 (2012)
Kinetics of antibody-dependent activation of the first component of complement on lipid bilayer membranes
Parce JW
Biochemical and Biophysical Research Communications, 93, 235-242 (1980)
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