S0389

Sucrose

for molecular biology, ≥99.5% (GC)

• Synonym(s): α-D-Glc-(1→2)-β-D-Fru, α-D-Glucopyranosyl β-D-fructofuranoside, β-D-Fructofuranosyl-α-D-glucopyranoside, D(+)-Saccharose, Sugar
• Empirical Formula (Hill Notation): C₁₂H₂₂O₁₁
• CAS Number: 57-50-1
• Molecular Weight: 342.30
• Beilstein: 90825
• EC Number: 200-334-9
• MDL number: MFCD00006626
• UNSPSC Code: 12352201
• PubChem Substance ID: 24899423
• NACRES: NA.31

Properties

• biological source: sugar cane
• Quality Level: 300
• grade: for molecular biology
• Assay: ≥99.5% (GC)
• form: crystals
• technique(s): HPLC: suitable; cryopreservation: suitable; electron microscopy: suitable; enzyme immunoassay: suitable; gas chromatography (GC): suitable; immunohistochemistry: suitable
• impurities: ≤0.1% free glucose
• color: white
• useful pH range: 5.5-7 (25 °C, 342 g/L)
• mp: 185-187 °C (lit.)
• solubility: H₂O: soluble 50 g + 50 mL
• cation traces: heavy metals (as Pb): ≤5 ppm
• absorption: ≤0.15 at 280 at 50%; ≤0.20 at 260 at 50%
• suitability: suitable for molecular biology
• application(s): agriculture; agriculture; life science and biopharma; metabolomics; sample preservation
• foreign activity: DNase, RNase, protease, none detected
• storage temp.: room temp
• SMILES string: OC[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
• InChI: 1S/C12H22O11/c13-1-4-6(16)8(18)9(19)11(21-4)23-12(3-15)10(20)7(17)5(2-14)22-12/h4-11,13-20H,1-3H2/t4-,5-,6-,7-,8+,9-,10+,11-,12+/m1/s1
• InChI key: CZMRCDWAGMRECN-UGDNZRGBSA-N

Description

General description

Sucrose, a disaccharide composed of glucose and fructose, serves as an energy source in various biological processes. In cell biology, sucrose acts as a cryoprotectant, preserving cell viability during freezing and thawing. It is also added as a supplement in culture media, aiding in maintaining osmotic balance and enhancing cell survival.In molecular biology, sucrose is crucial in gradient solutions for DNA and RNA purification and in density centrifugation for organelle separation.

Application

Sucrose has been used:

• to perform cryoprotection for kidney tissues
• as a component in the solid germination medium to germinate pollen grains
• in the triphenyl-tetrazolium chloride (TTC) test to check the viability of pollen grains
• in the cryoprotection of fixed brain tissues for immunohistochemistry

Biochem/physiol Actions

Sucrose is used as a transport and storage molecule in most plants due to its limited chemical reactivity. It can be used to prepare density gradients for the separation of cell/organelle. Sucrose can be used in the plant, insect, and bacterial culture media as a supplement. In addition, sucrose can also be used in several enzymatic assays.

Features and Benefits

• Ideal for Molecular Biology, Cell Biology and Biochemical research
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Tested for DNase, RNase, and proteases
• Molecular biology grade sucrose, sourced from sugar cane

Other Notes

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For additional information on our range of Biochemicals, please complete this form.

To gain a comprehensive understanding of our extensive range of Disaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves