B6429

Casein Blocking Buffer 10x

for Northern and Southern blotting, solution

• Synonym(s): 10x Blocking Buffer
• UNSPSC Code: 12161700
• NACRES: NA.54

Properties

• grade: for molecular biology
• form: solution
• technique(s): Southern blotting: suitable
• storage temp.: room temp

Description

General description

Casein is a phosphoprotein found in milk. It is classified into four subtypes, including αS1-, αS2-, β-, and κ-caseins.
Sigma’s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes.

Application

Casein Blocking Buffer 10x has been used in:

• western blotting
• enzyme linked immunosorbent assay (ELISA)
• immunohistochemistry

Features and Benefits

• Better blocking performance than dried milk or BSA
• Easy-to-use concentrate
• Compatible with neutral and positively-charged membranes
• Compatible with chemiluminescent and colorimetric detection assays

Preparation Note

Dilute 10x Blocking Buffer with molecular biology grade water before use.

Safety Information

• Pictograms: GHS07,GHS09
• Signal Word: Warning
• Hazard Statements: H315,H317,H319,H411
• Precautionary Statements: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable