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581324-U

Supelco

Ascentis® C18 (5 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

Sinonimo/i:

Ascentis RP18 HPLC Column

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About This Item

Codice UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Colonna per HPLC Ascentis® C18, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

Materiali

stainless steel column

Livello qualitativo

agenzia

suitable for USP L1 (Similar to Phenomenex Luna C18)

Nome Commerciale

Ascentis®

Caratteristiche

endcapped

Produttore/marchio commerciale

Ascentis®

Confezionamento

1 ea of

Grado di funzionalizzazione

25% Carbon loading

Parametri

≤70 °C temp. range
400 bar pressure (5801 psi)

tecniche

HPLC: suitable
LC/MS: suitable

Lungh. × D.I.

15 cm × 4.6 mm

Area superficiale

450 m2/g

Copertura della superficie

3.7 μmol/m2

Impurezze

<5 ppm metals

Matrice

fully porous particle
silica gel high purity, spherical

Gruppo funzionale matrice

C18 (octadecyl) phase

Dimensione particelle

5 μm

Dimensione pori

100 Å

operating pH range

2-8

applicazioni

food and beverages

Tecnica di separazione

reversed phase

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Descrizione generale

La famiglia di colonne Ascentis rappresenta la quarta generazione nella tecnologia delle colonne HPLC realizzate dai ricercatori Supelco. Tutte le colonne Ascentis sono caratterizzate da silice da 100 Angstrom a elevata purezza con particelle da 3, 5 e 10 micron. Le colonne sono progettate per applicazioni su piccole molecole e sono adeguabili a varie dimensioni, da quelle delle microcolonne (DI di 1,0 mm) a quelle preparatorie (DI di 50 mm). La famiglia include fase C18, C8, fenil, Si e con gruppo polare integrato, RP-Amide.

Ascentis C18 è una colonna HPLC di prima scelta estremamente stabile e affidabile che fornisce una forma a picchi simmetrici e un′ottima ritenzione anche con composti difficili.

Applicazioni


  • Preparation of polar embedded C18 stationary phase for efficient separation of peptides and proteins in high performance liquid chromatography.: This study details the development of a polar embedded C18 stationary phase, demonstrating its effectiveness in separating peptides and proteins. The Ascentis® C18 HPLC Column was utilized for its high separation efficiency, crucial for proteomics and biopharmaceutical applications (Ali et al., 2022).

  • A dilute-and-shoot liquid chromatography-tandem mass spectrometry method for urinary 18-hydroxycortisol quantification and its application in establishing reference intervals.: Researchers used the Ascentis® C18 HPLC Column to develop a sensitive method for quantifying urinary 18-hydroxycortisol. This method aids in clinical diagnostics and endocrinological studies by providing accurate reference intervals (Zhao et al., 2022).

  • A Comparative Study on Analysis of Ginsenosides in American Ginseng Root Residue by HPLC-DAD-ESI-MS and UPLC-HRMS-MS/MS.: This comparative analysis utilized the Ascentis® C18 HPLC Column to separate and identify ginsenosides in American ginseng. The study highlights the column′s application in natural product research and quality control in the pharmaceutical industry (Hsu et al., 2022).

  • Validation of a Rapid and Easy-to-Apply Method to Simultaneously Quantify Co-Loaded Dexamethasone and Melatonin PLGA Microspheres by HPLC-UV: Encapsulation Efficiency and In Vitro Release.: This research validates an HPLC method using the Ascentis® C18 Column to simultaneously quantify dexamethasone and melatonin in PLGA microspheres, offering a robust tool for drug delivery studies (Brugnera et al., 2022).

  • Simultaneous determination of ivermectin, clorsulon and their related substances in an injectable finished product by a stability-indicating RP-HPLC method.: The Ascentis® C18 HPLC Column was employed to develop a stability-indicating method for analyzing ivermectin and clorsulon in pharmaceutical formulations, emphasizing its role in quality control and regulatory compliance (Padivitage et al., 2022).

Caratteristiche e vantaggi

  • Ottima ritenzione
  • Forma a picchi simmetrici
  • Elevata riproducibilità
  • Totale compatibilità LC-MS

Prodotti consigliati

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Note legali

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Dipanjan Goswami et al.
Biomedical chromatography : BMC, 23(11), 1227-1241 (2009-07-14)
A LC-MS/MS method for plasma topiramate analysis is delineated involving least number of healthy volunteers. Topiramate and amlodipine internal standard (IS) were extracted by simple centrifuge-coupled solid-phase extraction and reverse-phase chromatographic separation was performed on an Ascentis C(18) column. Turbo-spray
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 55(5), 934-948 (2011-04-19)
In this study, the composition of polyphenols (phenolic acids and flavonoids) in propolis extracts was investigated by HPLC-DAD and HPLC-ESI-MS/MS by comparing the performance of ion trap and triple quadrupole mass analyzers. The analyses were carried out on an Ascentis
Federica Vacondio et al.
Journal of pharmaceutical and biomedical analysis, 46(1), 200-205 (2007-10-26)
A rapid, simple and sensitive liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for the determination of the imidazole H(3) antagonist ROS203 in rat plasma, using the superior homologue ROS287 as internal standard. Analyses were performed on an Agilent
Federica Pellati et al.
Journal of chromatography. A, 1242, 43-58 (2012-05-09)
In this study, a detailed phytochemical characterization of Echinacea pallida (Nutt.) Nutt. root extracts and dietary supplements was carried out for the first time by developing advanced chromatographic techniques, based on HPLC with diode array (DAD) and electrospray ionization-mass spectrometry
Meiyun Shi et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 951-952, 129-134 (2014-02-22)
Aralia mandshrica is a well-known traditional Chinese medicine from Northeast China commonly used to treat digestive, circulatory and immune system disorders. Calenduloside E is one of its bioactive components currently under evaluation as a pure drug. In this study, a

Articoli

Phosphonates are a class of compounds commonly used for therapeutic applications of antiviral drugs. These compounds can be difficult to analyze due to their highly polar nature and lack of UV active chromophores.

Protocolli

Because reversed-phase HPLC is primarily dependent on hydrophobic interactions between the stationary phase and analyte, ion pairing is occasionally necessary to obtain sufficient retention of polar, ionizable compounds.

HPLC Analysis of Warfarin™ Anticoagulant on Ascentis® C18

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Chromatograms

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