565302-U
Ascentis® RP-Amide HPLC Column
3 μm particle size, L × I.D. 15 cm × 2.1 mm
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About This Item
Prodotti consigliati
Materiali
stainless steel column
Livello qualitativo
agenzia
suitable for USP L60
Nome Commerciale
Ascentis®
Caratteristiche
endcapped
Produttore/marchio commerciale
Ascentis®
Confezionamento
1 ea of
Grado di funzionalizzazione
19.5% Carbon loading
Parametri
≤70 °C temp. range
400 bar pressure (5801 psi)
tecniche
HPLC: suitable
LC/MS: suitable
Lungh. × D.I.
15 cm × 2.1 mm
Area superficiale
450 m2/g
Copertura della superficie
2.7 μmol/m2
Impurezze
<5 ppm metals
Matrice
fully porous particle
silica gel high purity, spherical
Gruppo funzionale matrice
amide, alkyl phase
Dimensione particelle
3 μm
Dimensione pori
100 Å
operating pH range
2-8
applicazioni
food and beverages
Tecnica di separazione
reversed phase
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Descrizione generale
The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.
Ascentis RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis RP-Amide is the first choice in embedded polar group HPLC phases.
Ascentis RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis RP-Amide is the first choice in embedded polar group HPLC phases.
Caratteristiche e vantaggi
- Excellent retention and peak shape for polar compounds
- 100% aqueous compatibility
- Ultra low bleed, LC-MS compatible
- Unique selectivity
Note legali
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Precolonna
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Journal of pharmaceutical analysis, 3(6), 489-499 (2013-12-01)
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used for simultaneous quantification of tolterodine and its metabolite 5-hydroxy methyl tolterodine in rat plasma. Tolterodine-d6 and 5-hydroxy methyl tolterodine-d14 were used as internal standards (IS). Chromatographic separation was performed on Ascentis Express
Journal of pharmaceutical and biomedical analysis, 151, 291-300 (2018-02-08)
The presented work describes the development and validation of a rapid UHPLC-UV method using a fused core particle column with an RP-Amide stationary phase for the separation and quantitative analysis of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. Three
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